人乙型肝炎病毒实验感染红面猴初报

选用无自然感染人乙型肝炎病毒(HBV)的红面猴4只,其中2只实验感染人HBV后,进行血清学检测SGPT(改良赖氏法)、HBV标志物“两对半”(RPHA法、ELISA法、SPRIA法)、HBV-DNA(斑点分子杂交法)、乙肝病毒颗粒(电镜观察);并通过猴肝组织活检进行光、电镜动态病理学观察,以及用组织化学、免疫组化和原位杂交等法检测肝细胞内的HBsAg、HBcAg和HBV-DNA。实验猴SGPT、HBsAg、抗-HBc、抗-HBs、HBV-DVA和Dane颗粒阳性,其中HBsAg阳性持续时间为52和133周;并发现猴肝组织由急性肝炎发展为慢迁肝的病变,与乙肝病人的病变相同,同时在肝细胞内检出HBV标志物。

A Report on Experimental Infection of Human Hepatitis B. Virus in Macaca speciosa

Four Macaca speciosa without infection HBV naturely were selected for this study. Two of them used as HBV infection. Two another for the contral. After this, the sera of animals were drawn once for every 2 to 4 weeks in the first year, and it drawn once for every 2 to 4 months in the second year. The positive of SGPT , HBV markers, HBV-DNA , Dane particles were observed by R-F, RPHA , ELISA, SPRIA , Hybridization spot test and EM respectively. The HBsAg-positive lasted for 52 (1 # ) and 133 (8# ) weeks, Performing liver biopsy in various period, and speciments were observed for HBSAg , HBeAg and HBV-DNA by light microscope, EM , histochemistry, immunohis-tochemistry and in situ hybridization. The results showed that two animals developed into acute to chronic hepatitis B and their feature to resemble acute and chronic hepatitis B in human. We also inoculated sera from 1 # and 8# into 13 # which the SGPT and HBsAg stronger positive at 28 weeks. But it died for dysentery at 8th days, so that observe ation stoped.