| 捕捉ABC—ELISA检测肺结核病人特异性CIC方法的建立 |
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| 引用本文： | 伍学洲 程黎莉. 捕捉ABC—ELISA检测肺结核病人特异性CIC方法的建立[J]. 中国人兽共患病杂志, 1991, 7(3): 34-36 |
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| 作者姓名： | 伍学洲 程黎莉 |
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| 作者单位： | 江西中医学院微生物学教研室(伍学洲，苏东明)，南昌市红十字会中心血站(程黎莉)，南昌市红十字会中心血站(吴钟玲) |
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| 摘 要： | 利用抗人IgG及抗人IgA捕捉完整的结核特异性IgG型及IgA型循环免疫复合物(CIC),进而用抗结核菌抗体检测的原理,建立起捕捉ABC-ELISA检测肺结核病人特异性CIC方法。对100例肺结核病人检测IgG型及IgA型CIC分别为35％及73％(P<0.001),总阳性率为85％;对40例正常人非特异反应IgG型及IgA型CIC分别为2.5％及7.5％(P>0.05),总特异性为90％。结果既证明结核病人血清中确含完整的结核特异性CIC,又为检测之提供了一套候选方法。
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| 关 键 词： | 结核病 循环免疫复合物 IgG型及fgA型 捕捉ABC-ELISA检测法 |
Establishment of the Method for Detecting Specific Circle Immune Complex in the Sera of Plumonary Tuberculosis with Capture ABC-ELISA |
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| Wu Xuezhou et al. Establishment of the Method for Detecting Specific Circle Immune Complex in the Sera of Plumonary Tuberculosis with Capture ABC-ELISA[J]. Chinese Journal of Zoonoses, 1991, 7(3): 34-36 |
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| Authors: | Wu Xuezhou et al |
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| Affiliation: | Wu Xuezhou et al |
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| Abstract: | The method of Capture-ABC (Avidin-Biotin-HRP-Complex) ELISA for detecting specific circle immune complex (CIC) in the sera of plumonary tuberculosis has been established In 100 cases of plumonary tuberculoses, 35% had IgG type of specific CIC and 73% had IgA type of specific CIC (P<0. 001). The positive rate of whole specific CIC was 85%. In 40 cases of normal controls, the non-specific reactions were as follow: IgG type (2.5%), IgA type (7.5%), P>0. IgG type (2. 5%), IGA type (7. 5%), P>0. 05. The specificity was 90%. |
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| Keywords: | Tuberculosis Circle immune complex TgG type and IgA type Capture-ABC-ELISA. |
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