| 用荧光原位杂交(FISH)方法研究人类卵巢癌和胃癌细胞系17号染色体拷贝数变化 |
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| 引用本文： | 郑淑芳,乔惠珍,郭美君,路桂荣,冯莉雅,宁涛,吕有勇,柯杨. 用荧光原位杂交(FISH)方法研究人类卵巢癌和胃癌细胞系17号染色体拷贝数变化[J]. 肿瘤研究与临床, 1999, 0(1) |
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| 作者姓名： | 郑淑芳 乔惠珍 郭美君 路桂荣 冯莉雅 宁涛 吕有勇 柯杨 |
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| 作者单位： | 北京市肿瘤防治研究所，内蒙古医学院第一附属医院 |
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| 基金项目： | 国家自然科学基金,“863”国家重点攻关项目资金 |
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| 摘 要： | 目的研究载有多种癌变相关基因的１７号染色体在不同器官、不同组织类型肿瘤单个细胞内拷贝数的变化规律。方法以正常外周血淋巴细胞为对照，采用ＦＩＳＨ方法检测各细胞株１７号染色体拷贝数。结果１７号染色体在各细胞株单个细胞中拷贝数表现为：ＳＫＯＶ３以六倍体为主，占４８％，七倍体率占２３％；３ＡＯ以三倍体为主，占９３％；ＯＶ２以四倍体为主，占６７％；ＭＧＣ８０３以六倍体为主，占６４％；ＢＧＣ８２３以二倍体为主，占８３％；ＳＧＣ７９０１以三倍体为主，占８０％；ＰＡＭＣ８２以五倍体为主，占７８％。结论各细胞系均无１７号染色体缺失，反呈扩增趋势。
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| 关 键 词： | 卵巢癌 胃癌 细胞株 FISH 17号染色体 |
COPY NUMBER CHANGE OF CHROMOSOME 17 IN HUMAN OVARIAN OR GASTRIC CANCER CELL LINE BY FLUORESCENCE IN SITU HYBRIDIZATION |
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| Zheng Shufang,Qiao Huizhen,Guo Meijun Beijing Insitute for Cancer Research. COPY NUMBER CHANGE OF CHROMOSOME 17 IN HUMAN OVARIAN OR GASTRIC CANCER CELL LINE BY FLUORESCENCE IN SITU HYBRIDIZATION[J]. Cancer Research and Clinic, 1999, 0(1) |
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| Authors: | Zheng Shufang Qiao Huizhen Guo Meijun Beijing Insitute for Cancer Research |
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| Abstract: | Objective To study the copy number change at chromosome 17 in different tumor cell lines.Methods Fluorescence in situ hybridization (FISH) was used to test the copy number in individual ovarian cancer and gastric cancer cells in vitro .The normal peripheral lymphocytes were used as controls. Results chromosome 17 in SKOV3 is hexaploid (48%),seven ploid(23%),tirploid (93%)in 3AO,tetropoid (67%)in OV2,hexa ploid (64%) in MGC803,biploid(83%) in BGC823.Conclusion There is no chromosome 17 deletion in most cell lines studied. On the contrary, it showed a tendency of amplification.Further study is needed for the understanding of the significance of the results. |
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| Keywords: | Ovarian cancer Gastric cancer Cell line FISH Chromosome17 |
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