完全脱蛋白骨复合rhBMP2构建人工骨及其体内异位成骨

目的: 探讨异种完全脱蛋白骨复合基因重组骨形态发生蛋白(rhBMP2) 在体外构建人工骨的可行性,并对其体内异位成骨进行评估。方法: 体外将兔骨髓基质细胞进行成骨诱导, 然后接种复合rhBMP2的小牛完全脱蛋白骨, 构建组织工程骨, 植入自体大腿肌袋内, 术后4、8周处死动物分别进行常规组织学检查、碱性磷酸酶活性测定、折弯力学测试, 观测这种组织工程骨在体内的异位成骨作用。结果: 这种方法构建和组织工程骨在体内异位成骨效应显著且抗折能力较强。结论: 异种完全脱蛋白骨复合rhBMP2可作为骨组织工程支架材料, 这种组织工程骨在体内的成骨能力随植入时间的延长而增加。

Tissue-engineered artificial bone regeneration in vitro and its effect of ectopic osteogenesis in vivo

Objective:To study the feasibility of tissue-engineered artificial bone regeneration in vitro,and evaluate effect of ectopic osteogenesis after implantation in vivo.Method:Fully compound deproteinized bone(CFDB) treated physically and chemically were composited with recombination human bone morphogenetic protein-2(rhBMP2),then,the authors constructed the tissue-engineered artificial bone with osteoblasts-derived from self mesenchymal stem cells (MSCs) and implanted in rabbit,the effect of osteogensis was studied through hematoxylin and eosin staining,alkaline phosphatase activity assay,bent test respectively.Result:The effect of osteogenesis of the tissue-engineered artificial bone is evidential and its anti-bent ablity is better.Conclusion:Fully compound deproteinized bone composited with recombination human bone morphogenetic protein-2(rhBMP2) could be applied as the scaffold materials of bone tissue engineering,and,more new bone tissue forms gradually as the time went by after implantation.