A PCR–restriction fragment length polymorphism assay to genotype human metapneumovirus |
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| Authors: | M Montes D Vicente O Esnal G Cilla E Pérez-Trallero |
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| Institution: | Servicio de Microbiología, Hospital Donostia;, CIBER Enfermedades Respiratorias;and Departamento de Medicina Preventiva y Salud Pública, Basque Country University, San Sebastián, Spain |
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| Abstract: | Human metapneumovirus (hMPV) genotypes A and B show epidemiological and probably clinical differences. This report describes a fast and simple PCR–restriction fragment length polymorphism (PCR-RFLP) assay, involving digestion of the fusion protein gene with Tsp 509I, that allows lineages A1, A2, B1 and B2 to be distinguished. The assay should help in elucidating the epidemiology of hMPV, and possibly in predicting the severity of clinical infection. |
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| Keywords: | Fusion protein gene genotypes human metapneumovuris identification lineages PCR-RFLP |
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