猕猴桃根多糖的脱色工艺研究

目的：选定猕猴桃根粗多糖的脱色材料,确定脱色工艺条件。方法：建立猕猴桃根多糖的含量测定方法,以脱色率为指标,采用单因素试验法,通过优化与比较聚酰胺和DA201型大孔吸附树脂对猕猴桃根粗多糖的脱色作用。结果：聚酰胺对RACP中的色素具有较大的吸附容量和选择性,选定聚酰胺静态吸附法对猕猴桃根粗多糖进行脱色,脱色工艺条件为：多糖浓度2mg/mL,药脂比60mg∶1g,pH5,室温下吸附80min。以该工艺处理猕猴桃根粗多糖,脱色率可达78%,多糖保留率大于85%。结论：建立的猕猴桃根粗多糖的的脱色方法与工艺,简单、可行。

Study on Decolorization Process of Radix Actinidia Chinensis Polysaccharides

Objective：To select decoloring materials for the purification of RACP,and establish the technology conditions of decolorization process.Methods：To determine RACP by HPLC;The index is decolorate rate and haplo-factor method is adopted through comparing and optimizing the polyamide with DA201 gum on the decolorization of RACP.Results： The polyamide have more adsorptive capacity and more selectivity to the pigment.The conditions of static adsorption of Polyamide in decolorization we choosed were as follows： concentration of polysaccharide 2（mg / mL）,rationem of pharmacon and polyamide was 60mg∶1g,pH 5,adsorption at room temperature for 80mins,throug this process,the decolorization rate reached up to 78%,and the retention rate of RACP was greater than 85%.Conclusion：The method and technology of decolorization of RACP are simple and feasible.