地骨皮调控H2O2所致内皮细胞凋亡的有效部位及相关信号通路研究

目的：筛选地骨皮调控H2O2所致人脐静脉血管内皮细胞（human umbilical vein endothelial cells,HU-VEC）凋亡的有效部位,并研究相关的信号转导通路,探讨其保护作用的分子机制。方法：用H2O2构建凋亡模型,运用MTS检测细胞存活率,流式细胞仪检测凋亡率筛选地骨皮有效部位,及Western观察相关信号通路蛋白P-AKT、P-NF-KB表达情况。结果：地骨皮30%,50%,70%醉提取部位均可以减少HUVEC的凋亡,可以明显增加P-AKT的表达,降低P-NF-KB活性,尤其55%醇提地骨皮用量最小效果最佳。结论：地骨皮抑制H2O2所致HUVEC凋亡的主要成分在55%醇提部位,它可以通过AKT、NF-κB信号通路起到抗凋亡的作用。

The effective site of Cortex Lycii Radicis protects HUVEC from H2O2-induced apoptosis and Associated signaling pathway study

Objective： This study was performed to select the most effective site of Cortex Lycii Radicis that protect human umbilical vein endothelial cells（HUVEC） from H2O2-induced apoptosis and study the Associated signaling pathway to reveale the possible molecular mechanisms involved.Methods： Apoptosis model of HUVEC was established by inducing of H2O2.The apoptosis cells were assessed by MTS and Annexin V-propidium iodide double staining flow cytometry to select the effective site of CLR against H2O2-induced apoptosis.The activities of AKT NF-KB were assayed by Western blot to investigate the underlying molecular mechanisms.Results：Stimulated with H2O2.a high fraction of HUVEC underwent apoptosis.While in the presence of CLR（30%,50% and 70% alcohol extracts）.HUVEC apoptosis was blocked.The activation of AKT induced by H2O2 was significantly improved and the activation of NF-κB was significantly reduced,55% alcohol extract from CLR seemed to be more effective than 35% and 75% alcohol extract to protects HUVEC from H2O2-induced apoptosis.Conclusion： The 55% alcohol extract is the most effective site of CLR to protect HUVEC from H2O2-induced apoptosis through inhibition of the activation of AKT and NF-KB.