苏红透骨散对骨关节炎模兔去分化软骨细胞影响的实验研究

目的：探讨苏红透骨散对膝骨性关节炎模兔软骨细胞增殖、凋亡的影响。方法：60只新西兰大白兔用HuIth法造骨关节炎动物模型,随机分成空白对照组、实验组;实验组用苏红透骨散灌胃,空白对照组用生理盐水。治疗第4、8、12、24周后每组各取6只动物,用亚硝酸盐还原酶法测定关节滑液NO水平。关节软片组织HE染色,光镜观察形态学变化、电镜观察软骨细胞内结构变化及原位末端标记（TLNEL）法观察软骨细胞凋亡病理改变,进行组织炳理学评估。结果：空白对照组NO活性在不同时期均高于实验组（8周,P〈0.05;12、24周,P〈0.01）,两组间比较有显著性差异（P〈0.05,0.01）：实验组的关节软骨病理改变总积分、软骨细胞病理改变积分和软片表层病理改变积分均明显低于空白对照组（P〈0.05）;实验组的软骨细胞凋亡指数与对照组的软骨细胞凋亡有显著性差异（P〈0.05）,与关节液中NO含量显著相关。结论：苏红透骨散对兔膝关节实验性骨关节炎软骨退变具有保护作用,通过NO诱导途径减少软骨细胞的凋亡,可能是其作用机制之一。

Empirical Study of Effect Suhongtougu Granula to Rabbit Osteoarthritis Chondrocyte

Objective：Investigate Suhongtougu granula affecting chondrocyte multiplication apoptosis.Methods：60 rabbits were made model by Hulth,and divlaed two groups,vacant group and treating group.at 4weeks;8weeks;12weeks;24weeks,be executed and be draw the materials from rabbits.And chemical examining;observating.by light microscope and electron microscope.By in situ end-labeling observing chondrocyte apoptosis.（8 weeks P 〈 0.05,24weeks P 〈 0.01）.Conclusion ：Suhongtougu granula could protecting cataplasia chondrocyte,by NO inducing de-crease chondrocyte apoptosis.It maybe one mechanism of action.