不同证候小鼠下丘脑-垂体-甲状腺轴基因表达的特征

目的：观察不同证候小鼠下丘脑-垂体-甲状腺轴（HPTA）相关主要已知基因表达的特征。方法：采用小鼠计量化四诊和辨证方法、及GeneChip Mouse Exon 1.0 STArray等技术,观察正常偏气虚证小鼠、早期邪毒壅盛证和早期气虚证荷瘤小鼠HPTA相关基因表达差异。结果：（1）正常小鼠气虚证者,甲状腺机能减退,Atp1a1、Atp1a2、Atp1a3、Atp1b1、Atp1b2、Pax8、Tg、Tpo、NIS等与甲状腺素合成有关基因的表达水平一致下降;而垂体、下丘脑处于积极的代偿之中,TRH、TSH等激素表达增加。（2）肿瘤发生后的早期,荷瘤小鼠亦出现甲状腺机能的减退,垂体、下丘脑亦处于积极的代偿之中,TRH、TSH等激素表达增加。但是,该阶段邪毒壅盛证小鼠,垂体已出现失代偿的征兆,TSHb的表达量已显著低于同期的气虚证以及正常气虚小鼠。结论：甲状腺机能减退可能是气虚证发生的重要基础,而非下丘脑和垂体。荷瘤小鼠早期邪毒壅盛证垂体继发的失代偿表现,可能与该证候荷瘤小鼠带瘤生存期短、预后差有关。

Gene-Expression Signature in Hypothalamus-Pituitary-Thyroid axis of Mice with Different Symptoms and Signs

Objective： To analyses the Gene-expression signature in hypothalamus-pituitary-thyroid axis of H22 tumor mice with different symptoms and signs.Methods： By the quantitative four diagnosis and syndrome differentiation methods and Gene Chip Mouse Exon 1.0 ST Array,the related genes＇ expression difference were measured in qi-deficency syndrome of normal mice,qi-deficency syndrome in early stage,and poisonous pathogenic factors syndrome.Results：（1） there is hypo function in thyroid gland in qi-deficiency normal mice,expression of some genes related to the thyroxin synthesis decreased,including Atp1a1,Atp1a2,Atp1a3,Atp1b1,Atp1b2,Pax8,TG,Tpo,NIS,et al,while hypothalamus and pituitary were in aggressive compensatory,the expression of TRH and TSH increased.（2）In early stage after tumorigenesis,hypofunction in thyroid also existed in tumor-bearing mice,hypothalamus and pituitary were also in aggressive compensatory,the expression of TRH and TSH increased too.But poisonous pathogenic factors syndrome in this period,decompensation has been found,the expression of TSHb dropped significantly comparing the qi-deficency mice in same stage and in normal mice.Conclusion： hypofunction in thyroid gland may be the important material basis of qi-deficiency syndrome,while hypothalamus or pituitary is not.The secondary decompensation in pituitary with poisonous pathogenic factors syndrome in early period may be related with the shorter survival time and bad prognosis.