中药怀牛膝的液相色谱指纹图谱研究

目的：建立怀牛膝药材液相色谱指纹图谱检测方法,为其质量控制提供方法。方法：以蜕皮甾酮为参照物,采用RP-HPLC法,色谱柱为Kromasil-C18（250mm×4.6mm,5μm）,流动相为乙腈-0.4%磷酸水溶液梯度洗脱,检测波长为254nm。结果：该方法可使怀牛膝药材中各成分较好分离,并根据检测结果确定了14个共有指纹峰,建立了怀牛膝HPLC指纹图谱共有模式。结论：建立的指纹图谱方法简便可靠,可以作为怀牛膝药材质控的重要方法。

Fingerprint Chromatogram of Radix Achyranthes Bidentata

Objective： To establish detecting methods with fingerprint chromatogram for the Achyranthes bidentata. Methods： Kromasil- 5 C18 column （ 250 mm ×4.6 mm , 5μm ） was used with a mixture of acetonitrile- 0.4% phosphorus acid as mobile phase in a gradient mode, and with ecdysteron as the reference substance. The wavelength was 254nm. The data were analyzed with＇ Computer Aided Similarity Evaluation＇ software. Results： Established the typical fingerprint chromatogram. Conclusion： The method is simple and accurate with a good reproducibility and can be well used in quality control of Achyranthes bidentata.