哮喘豚鼠血红素氧合酶1的表达与调节

目的　探讨血红素氧合酶１（ＨＯ１） 在哮喘中的表达与作用机制。方法　７０ 只豚鼠完全随机分为７ 组，每组１０ 只，其中两组分别应用ＨＯ１ 特异性激动剂血晶素和抑制剂锡原卟啉处理豚鼠哮喘模型，另外５ 组分别为哮喘发作前、发作、自然缓解、地塞米松预防和正常对照组，应用分光光度计和放射免疫竞争分析法等，检测血及肺组织的ＨＯ１ 活性、一氧血红蛋白（ＣＯＨｂ）、环磷酸鸟苷（ｃＧＭＰ） 、血浆ＩｇＥ 水平，观察支气管肺泡灌洗液（ＢＡＬＦ） 中细胞数、肺组织病理学和ＨＯ１ 免疫组织化学染色的变化。结果　哮喘发作组和血晶素激动组的各项指标与正常对照组比较，差异均有非常显著性（ｔ＝４－７５４～１０－１８８，Ｐ＜０－０１） ，血晶素组更为显著，如肺ＨＯ１ 活性：每小时每毫克蛋白生成胆红素量为（１４４９±４２６）ｐｍｏｌ；肺ＣＯＨｂ：每毫克蛋白含（０－８３ ±０－２９） ％ ；肺ｃＧＭＰ：每毫克蛋白含（１－９６ ±０－６５）ｐｍｏｌ；血浆ＩｇＥ：（７３±２１） ｋＵ／Ｌ；ＨＯ１ 蛋白表达≥４ 级；ＢＡＬＦ细胞总数：（２１±４） ×１０８／Ｌ。地塞米松预防组和锡原卟啉抑制组除ＣＯ 含量和肺ＨＯ１ 活性略高外，余项指标与正常对照组比较差异无

The expression and regulation of heme oxygenase 1 in asthmatic guinea pigs

OBJECTIVE: To explore the expression and pathogenic mechanism of heme oxygenase-1(HO-1) in asthma. METHODS: Two groups of guinea pigs (10 in each) were treated with the specific stimulator(hemin) and inhibitor(Sn-PP) of HO-1 respectively. Their effects on the levels of HO-1 activity, COHb, cGMP and IgE in serum or blood and lung tissues were compared with each other. Acute asthmatic group (AAG), dexamethasone-prevented group(DPG), stable asthmatic group, ovalbumin-sensitized group and normal control group(NCG) were also included. Lung tissues of tested animals were pathologically observed and immunohistochemically stained. RESULTS: In the AAG and hemin stimulated group, the levels of COHb, cGMP and IgE were significantly increased with higher levels of activity and expression of HO-1 protein compared with the NCG (t = 4.575-10.188, P < 0.01). In DPG and Sn-PP inhibited groups, however, all measured parameters were markedly reduced compared with the AAG(P < 0.01). The other groups were normal. CONCLUSIONS: The increased production and activity of HO-1 resulted in increases of endogenous CO and cGMP and promoted inflammation and immunoreaction in asthma.