川芎嗪,丹参治疗大鼠肺纤维化对Ⅰ,Ⅲ型前胶原基因表达的影响

目的观察川芎嗪、丹参治疗大鼠肺纤维化对胶原基因表达的影响。方法大鼠随机分为正常对照、３个模型、川芎嗪、丹参和氢化可的松琥珀酸钠（氢可）组７个组。博莱霉素复制模型后１５～２８天给药，２９天取肺作ＨＥ染色及检测α１（Ⅰ）、α１（Ⅲ）前胶原ｍＲＮＡ的原位杂交，进行组织病理学检查及图像分析定量。结果模型组两种前胶原ｍＲＮＡ在第７天表达至高峰，２９天Ⅰ型仍保持高水平，Ⅲ型已恢复正常。经治疗川芎嗪组的α１（Ⅰ）前胶原ｍＲＮＡ降至正常（Ｐ＞０．０５），丹参、氢可组仍较高（Ｐ＜０．０１）。各组的α１（Ⅲ）前胶原ｍＲＮＡ均达正常（Ｐ＞０．０５）。结论川芎嗪通过抑制α１（Ⅰ）前胶原ｍＲＮＡ而起到抗肺纤维化作用，丹参和氢可的作用次之。

The therapeutic effect of ligustrazin and salvia miltiorrhiza on the gene expression of alpha1 (I) and alpha1 (III) procollagen in rat pulmonary fibrosis]

OBJECTIVE: To evaluate the effect of ligustrazin and salvia miltiorrhiza on collagen gene expression in rat pulmonary fibrosis (PF). METHODS: The tested animals were divided into 7 groups: normal control, untreated animal groups (day 7, 14 and 29), Ligustrazin, Salvia Miltiorrhiza and hydrocortisone groups. Treatment was started from day 15 to day 28. HE stain and in situ hybridization with alpha(1) (I) and alpha(1) (III) procollagen (PC) cDNA probes were applied to rat lungs on day 29. The results were quantified by a feature analysor. RESULTS: All the three drugs were effective, ligustrazin the best (P < 0.01), salvia miltiorrhiza the second (P < 0.01). In untreated groups both alpha(1) (I) and alpha(1) (III) PC mRNA reached the maximum on day 7. Alpha(1) (I) PC mRNA still kept high on day 29 while alpha(1) (III) PC mRNA had decreased to normal. In ligustrazin group alpha(1) (I) PC mRNA decreased to normal (P > 0.05) but in salvia miltiorrhiza and hydrocortisone groups it was still above normal level (P < 0.01). In all groups alpha(1) (III) PC mRNA had decreased to normal on day 29. CONCLUSIONS: Inhibitive effect of ligustrazin on alpha(1) (I) PC mRNA may play an important role in treating PF. The effect of salvia miltiorrhiza is less than ligustrazin.