脑脉康颗粒剂对大脑中动脉缺血再灌注大鼠脑的保护作用

探讨益气活血开窍复方脑脉康颗粒对缺血再灌注脑保护作用的机理.用线栓法制成大鼠脑缺血再灌注模型,采用全自动氨基酸分析仪、干湿重法、原子吸收分光光度计测量脑组织中兴奋性氨基酸(EAA)含量、含水量及钙离子(Ca2+)含量,HE法观察脑组织形态学改变.结果显示中药组谷氨酸 (Glu)含量下降(P<0.01),谷氨酸/ γ-氨基丁酸(Glu/ GABA)趋向于正常比值,脑组织含水量及Ca2+含量下降(P<0.05).中药可减轻由于缺血再灌注所导致的脑细胞形态学的改变.证明益气活血开窍方脑脉康颗粒通过抑制EAA释放,Ca2+内流而发挥脑保护作用.

Protective Effects of Naomaikang Granules on the Brain in the Rat Undergone Middle Cerebral Artery Ischemia and Reperfusion

The mechanism of the protective effects of Naomaikang Granules (NG), which are prepared according to a TCM formula for benefiting qi, promoting blood circulation and inducing resuscitation, on the brain were studied in the rat undergone middle cerebral artery ischemia and reperfusion. The rat model of ischemia and reperfusion was established by Longa's method. The levels of excitatory ammo acids (EAA), water content and the level of Ca+2 were measured in the brains of the rats by automatic amino acid analysis, dry and wet weight method and atomic absorption spectrophotometry. Rat cerebral morphologic changes were observed by HE method. The results showed that the level of Glu decreased (P < 0.01), the ratio of Glu/GABA tended to be normal, and both the water content and the level of Ca+2 decreased (P<0.05) in the brains in the rats in the NG-treating group. It was also found that the morphologic changes of neurons caused by ischemia and reperfusion were relieved in the NG-treating group. The results suggest that NG exerts its effects against cerebral injury through its inhibiting EAA release and Ca+2 intrinstic flow.