Analysis of isoenzymes in normal and carcinogen-treated human endometrial stromal cells in culture

The isoenzyme patterns of lactate dehydrogenase (LDH), hexokinase,phosphofructokinase, and aldolase were investigated in culturednormal and carcinogen-treated human endometrial stromal cells.Both normal and carcinogen-treated cells had similar phosphofructokinaseand aldolase isoenzymes. Distinctive changes in hexokinase andLDH isoenzyme patterns were found in the carcinogen-treatedstromal cells. The LDH isoenzyme patterns of the carcinogen-treatedstromal cells were shifted toward the muscle LDH forms. Thisis comparable to the alteration of LDH isoenzyme profiles observedin cell lines established from human uterine sarcomas. The twotissue culture media used affected the LDH isoenzyme patternsof endometrial stromal cells but differences between the LDHisoenzyme patterns of control and carcinogen-treated cells weredetected regardless of the growth medium used. Total LDH activitywas not significantly different in control and carcinogen-treatedstromal cells. The hexokinase isoenzyme patterns expressed bythe carcinogen-treated stromal cells were distinctly differentfrom the normal hexokinase patterns. The treated stromal cellscontained both hexokinase I and II, whereas the normal cellscontained only hexokinase I. Hexokinase and LDH isoenzyme patternsmay serve as markers with which to evaluate carcinogen-inducedneoplastic changes in cultured endometrial stromal cells.