不同高脂饲料对小鼠肥胖和胰岛素抵抗模型制备情况的效果比较

目的:探讨两种不同的高脂饲料在C57/BL6J小鼠肥胖和胰岛素抵抗(IR)模型制备过程中的效果差别,以期为更好的制备C57/BL6J小鼠肥胖和IR模型提供理论指导.方法:以雄性C57/BL6小鼠为实验材料,随机分为三组,分别为基础组,喂食国内A公司啮齿动物基础饲料;模型1组,喂食国内A公司生产的啮齿动物高脂饲料(HFD...

The effects comparison of different high fat diets on obesity and insulin resistance model in mice

Objective:To investigate the influence of two kinds of fat diets in the establishment of obesity and insulin resistance(IR)model in C57/BL6J mice,providing theoretical guidance for better obesity and IR models.Methods:Male C57/BL6J mice were inducted as materials and divided into three groups.The control group wasfed with the basal diets of rodentsfrom company A in ChinaModel group 1,fed with high-fat diets(HFD)from company A in China(HFD-A group).Model group 2,fed with HFD from Research Diets of American(HFD-R group).At the twelfth week,body weight,serum lipids,intraperitioneal glucose tolerance test(IPGTT)and intraperitionealinsulin tolerance test(IPITT)were detected and compared.Results:The average body weight of each group was increased with time.Until the twelfth week,the average body weight of control group was(31.781.36)g,HFD-A group was(41.561.67)g,and HFD-R group was(46.381.98)g,with significant difference(P<0.05).Until the twelfth week,the successful rate of obesity model was 100%(12/12)in both HFD-A and HFD-R group,without significance(P>0.05).Compared with control group,the levels of Glu,TG,CHOL,HDL,LDL and NEFA of HFD-R group were all changed significantly(P<0.05),however,only Glu and LDL elevated significantly in HFD-A group(P<0.05).Compared with control group,results of IPGTT and IPITT tests in HFD-R group were both enhanced significantly(P<0.05),while in HFD-A group,no significance was observed although with an increasing trend.Conclusion:For the obesity model,both HFDsin this study could establish the ideal model.However,only mice in HFD-R group meet the standard IR model at the same time.