长链非编码RNA CCHE1在胰腺导管腺癌中的表达及临床意义

目的 检测长链非编码RNA(lncRNA)CCHE1在胰腺导管腺癌(PDAC)细胞和组织中的表达,探讨lncRNA CCHE1与PDAC临床病理特征及预后间的关系.方法 RT-PCR检测PDAC组织及配对癌旁组织以及PDAC细胞和胰腺导管上皮细胞中lncRNA CCHE1的表达;利用单因素方差分析探讨lncRNA CC...

Clinical significance of long non-coding RNA CCHE1 in pancreatic ductal adenocarcinoma

Objective To investigate the expression level of long non-coding RNA cervical carcinoma high-expressed 1(lncRNA CCHE1)in pancreatic ductal adenocarcinoma(PDAC)cell and tissue and evaluate its prognostic value in PDAC.Methods PDAC tissue as well as adjacent normal tissue and PDAC cells and pancreatic ductal epithelial cells from patients diagnosed with PDAC and PDAC cells were harvested in this study.Expression of lncRNA CCHE1 was measured by real-time PCR.ANOVA analysis was performed to evaluate the association between lncRNA CCHE1 and clinical manifestation of PDAC patients.K-M survival analysis was utilized to determine correlation of PDAC expression with 5-year survival.Cox's proportional hazards regression models were applied to analyze the relationships between lncRNA CCHE1 and prognosis of PDAC patients.Results lncRNA CCHE1 was up-regulated in PDAC cells and tissues.Expression of lncRNA CCHE1 was closely associated with tumor differentiation and TNM stage of PDAC(P<0.05).Expression of lncRNA CCHE1 was negatively correlated with disease free survival and overall survival.Conclusion Expression of lncRNA CCHE1 is negatively associated with survival outcome of PDAC.lncRNA CCHE1 is an independent prognostic risk factor of PDAC.