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| 甲磺酸伊马替尼抑制黑素细胞游走的实验研究 | |
| 引用本文： | 夏建新,占部和敬,吴珊,王劲风,古江增隆.甲磺酸伊马替尼抑制黑素细胞游走的实验研究[J].中国皮肤性病学杂志,2008,22(1):1-3. |
| 作者姓名： | 夏建新 占部和敬 吴珊 王劲风 古江增隆 |
| 作者单位： | 1. 吉林大学第二医院皮肤科,吉林,长春,130041 2. 日本九州大学医学部皮肤科,日本,福冈 3. 吉林大学基础医学院病理生物学教研室,吉林,长春,130021 |
| 基金项目： | 吉林省科技厅科研项目 , 教育部留学回国人员科研启动基金 |
| 摘 要： | 目的探讨甲磺酸伊马替尼对人黑素细胞(NHEM)游走的抑制作用。方法培养NHEM,用四甲基偶氮唑蓝(MTT)还原法测定甲磺酸伊马替尼对NHEM生长和分化的影响;使用侵袭小室测定甲磺酸伊马替尼抑制NHEM的游走情况;用蛋白印记法测定甲磺酸伊马替尼抑制c-kit磷酸化情况。结果甲磺酸伊马替尼0.1μmol/L,1.0μmol/L,5.0μmol/L能够抑制由SCF50ng/mL引起的NHEM游走,并能抑制c-kit的磷酸化。结论甲磺酸伊马替尼抑制SCF引起的NHEM游走及抑制c-kit的磷酸化,为其用于防治色素痣治疗后的复发奠定了理论基础。 |
| 关 键 词： | 甲磺酸伊马替尼 游走 黑素细胞 |
| 文章编号： | 1001-7089（2008）01-0001-03 |
| 收稿时间： | 2007-05-29 |
| 修稿时间： | 2007-08-08 |
Study on the Inhibition of Normal Human Epidermal Melanocyte Migration by Imatinib Mesylate |
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| XIA Jian-xin, URABE Kazunori, WU Shan,et al.Study on the Inhibition of Normal Human Epidermal Melanocyte Migration by Imatinib Mesylate[J].The Chinese Journal of Dermatovenereology,2008,22(1):1-3. | |
| Authors: | XIA Jian-xin URABE Kazunori WU Shan |
| Abstract: | Objective To investigate the role of Imatinib mesylate on inhibition of normal human epidermal melanocyte(NHEM) migration.Methods The growth and the differentiation of NHEM were observed by MTT assay.Invasion-chamber was applied to determine the ability of NHEM's migration.Finally phosphorylation of c-kit in NHEM was studied by western blot.Results When the concentrations of Imatinib mesylate in culture medium were reach to 0.1 μmol/L,1.0 μmol/L and 5.0 μmol/L respectively,Imatinib mesylate inhibited NHEM migration that was induced by SCF and Imatinib mesylate also inhibited phosphorylation of c-kit in NHEM.Conclusion Imatinib mesylate can inhibit NHEM migration that was induced by SCF and Imatinib mesylate can also inhibit phosphorylation of c-kit in NHEM.This suggests that Imatinib mesylate may be potential used for prevention of nevus spilus recurrence. |
| Keywords: | Imatinib mesylate Migration Melanocyte |
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