黄芪及不同蛋白饮食对肾病综合征大鼠蛋白代谢及疗效作用研究

目的了解黄芪及不同蛋白浓度饮食对肾病综合征(NS)大鼠蛋白代谢及肾脏结构与功能的影响。方法用阿霉素制成大鼠NS模型,随机分为高、中、低蛋白饮食组(A、B、C)每组分为治疗组及对照组,治疗组每日以黄芪煎剂灌服,对照组以自来水2ml灌服,整个实验周期19周。结果喂黄芪4个月时,血白蛋白、肾对白蛋白排泌率有显著性的差异,肾病理:经黄芪治疗,光镜下系膜细胞减少,电镜下足突融合减轻,电子致密物沉着减少;高蛋白饮食组肾组织学损害重,低蛋白饮食组肾组织学损害轻。结论①黄芪虽然不减少尿蛋白总量排出,但可使血白蛋白升高,肾对白蛋白排泌率降低,使肾脏组织病理学损害减轻;②长期高蛋白摄入可加重蛋白尿,使肾功能损害加重,促进肾小球硬化;长期低蛋白饮食可使尿蛋白排泄量减少,对肾功能及肾组织学损害具有保护作用。

The Therapeutic Action and Protein Metabolism of Astrgalus.and different Protein diet on Rats with Nephrotic syndrom

Objective To determine the protein metabolism and effect on kidney' s structure and function cf astrgalus and different protein diet on rats with nephrotic syndrome.Methods The rats were randomly divided into high protein diet group (A),normal protein diet group (B),low protein diet group (C) and control group,treated group are given decocted liquid of astragalus everyday.The controlled group are given runnimg water daily (2ml).The whole experimental period is 19 weeks.Results At time of 4 months after taking astragalus,albumin and plasma creatining and ureanitrogen of treated group is compared with normal control have remarkable difference (P<0.01),Renal pathology:After the treatment of astragalus,mesangial cells were decreased in the optics microscope,and the foot process fusions were lightened and eletron dense deposits were decreased in the electron mircoscope;High-protein diet canaggravate the kidney damage in pathological sensa,but low protein diet can alleviate it.Conclusions (1)After admininstering with astrgalus,there is no reduction in excretion of proteinuria with there is a decrease in the renal excretion to albumin,an increase in albuminenia,a relief of kidney damage in pathlogical sense;(2)Long term high protein diet can aggravate proteinuria and damage of the function of the kidney,promote glomerular sclerosed.long term low protein diet can reduce quantity of proteinura and protect the function of kidney and damage of renal histology.