次声暴露对小鼠睾丸细胞超微结构的影响

目的 :通过对次声暴露下雄性小鼠睾丸细胞超微结构的变化情况 ,研究次声对雄性小鼠生殖的影响。　方法 :将 12只BALB C雄性小鼠随机分为暴露 1d组、7d组和 14d组 ,每组各取 1只小鼠分别暴露于 8Hz 90dB、8Hz 130dB、16Hz 90dB、16Hz 130dB的任一声压场中 ,2h d。对照组 3只小鼠 ,分别关在次声舱内 1、7、14d ,2h d ,但无次声作用。暴露结束当天处死 ,取睾丸固定 ,透射电镜下观察睾丸细胞超微结构的变化情况。　结果 :1d组中 ,小鼠睾丸支持细胞、间质细胞及生精细胞出现不同程度的细胞急性变性 ,细胞水肿 ,线粒体肿胀、空泡化 ,溶酶体增多 ,细胞坏死。损伤以次声参数为 8Hz 130dB最为严重。 7d组和 14d组中 ,出现了大量的畸形精子。　结论 :次声暴露可致睾丸细胞形态及功能受损 ,最终将影响小鼠的生育能力

Effects of Infrasound on Ultrastructure of Testis Cell in Mice

Objectives: To investigate the effects of infrasound on ultrastructure of testis in mouse. Mothods: Twelve male BALB/C mice were randomly divided into three groups according to exposed duration on 1,7 and 14 day. The mice were separately exposed to infrasound environment under 8 Hz/90 dB, 8 Hz/130 dB,16 Hz/90 dB,16 Hz/130 dB 2 hours per day. There was another control group which had three mice were separated into module with no infrasound. All the mice were killed on schedule. Then all the sections of testis were observed under electronic microscope. The alterations of structure and the chromatin were observed. Results: Some acute alteration in one day group was found in testis cell,such as cellular denaturation and necrosis,intercellular edema,mitochondria swelling,liposome hyperplasia.When the infrasound was up to 8 Hz/130 dB, the damage showed seriously. In 7 and 14 day group,the acute alteration was gradually decreased. A plenty of abnormal sperm were found. And other alteration was chromatin condense. The effect of variational frequency was impotant in ultrastructure. Conclusions: The infrasound markedly effected to testicular cell morphology and secreting function. Infrasound will lead to the alteration of procreation in mouse.