| 姜黄素逆转人结肠癌细胞株HCT-8/VCR多药耐药的研究 |
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| 引用本文： | 覃勇,傅仲学,卢伟东,李雷,汤为学.姜黄素逆转人结肠癌细胞株HCT-8/VCR多药耐药的研究[J].中国生化药物杂志,2011,32(3). |
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| 作者姓名： | 覃勇 傅仲学 卢伟东 李雷 汤为学 |
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| 作者单位： | 1. 重庆医科大学,附属第一医院普通外科,重庆400016
2. 重庆医科大学,病理生理学教研室,重庆400016 |
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| 基金项目： | 重庆市卫生局中医药重点项目 |
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| 摘 要： | 目的探讨姜黄素对人结肠癌耐药细胞株HCT-8/VCR多药耐药性的逆转作用及可能机制。方法运用MTT法进行药敏试验,流式细胞仪检测细胞内罗丹明123(Rh123)的表达,RT-PCR法检测多药耐药基因mdr1 mR-NA水平的变化,Western blot法检测mdr1基因蛋白产物P糖蛋白(P-gp)表达水平的变化。结果经25μmol/L姜黄素处理后,增强了HCT-8/VCR细胞对VCR的敏感性,其逆转倍数为4.58倍,增加了细胞内Rh123的蓄积(P<0.05),明显抑制了多药耐药基因mdr1 mRNA及其蛋白的表达(P<0.05)。结论本研究结果提示姜黄素能抑制耐药基因的表达及功能,提高细胞对化疗药物的敏感性,从而逆转结肠癌细胞的多药耐药性。
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| 关 键 词： | 姜黄素 结肠癌 多药耐药 逆转 |
Experimental study of multidrug-resistance reversing of curcumin on human colon carcinoma HCT-8/VCR cells |
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| QIN Yong,FU Zhong-xue,LU Wei-dong,LI Lei,TANG Wei-xue.Experimental study of multidrug-resistance reversing of curcumin on human colon carcinoma HCT-8/VCR cells[J].Chinese Journal of Biochemical Pharmaceutics,2011,32(3). |
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| Authors: | QIN Yong FU Zhong-xue LU Wei-dong LI Lei TANG Wei-xue |
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| Institution: | QIN Yong1,FU Zhong-xue1,LU Wei-dong1,LI Lei1,TANG Wei-xue2(1.Department of General Surgery,2.the No.1 Affiliated Hospital,Chongqing Medical University,Chongqing 400016,China) |
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| Abstract: | Purpose To investigate the multidrug-resistant reversing effect and its possible mechanism of curcumin on human colon carcinoma HCT8/VCR cells.Methods Drug sensitivity tests were analyzed by MTT method,flow cytometry(FCM) was applied to detect the expression of cell rhodamine 123(Rh123),RT-PCR assay was applied to determine the changes of the mdr1 mRNA of multidrug-resistance gene and Western blot assay was applied to test the changes of the expression of the mdr1 gene products P-gp.Results After treatment ... |
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| Keywords: | curcumin colon carcinoma multidrug resistance reverse |
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