Crystallization and Preliminary Structural Analysis of Dihydrolipoyl Transsuccinylase, the Core of the 2-Oxoglutarate Dehydrogenase Complex |
| |
| Authors: | David J Derosier Robert M Oliver and Lester J Reed |
| |
| Institution: | Clayton Foundation Biochemical Institute, University of Texas at Austin, Austin, Texas 78712;Department of Chemistry, University of Texas at Austin, Austin, Texas 78712 |
| |
| Abstract: | Dihydrolipoyl transsuccinylase, one of the three enzymes comprising the Escherichia coli 2-oxoglutarate dehydrogenase (EC 1.2.4.2) complex, has been crystallized. Studies by x-ray diffraction and electron microscopy establish that the transsuccinylase has octahedral (432) symmetry, i.e., it consists of 24 subunits that are structurally identical. |
| |
| Keywords: | |
|
|
