| 蓝光试验、潜血试验和ATP生物荧光法测定血液残留灵敏度的比较研究 |
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| 引用本文： | 邢书霞,马玲,王志,张流波,李新武. 蓝光试验、潜血试验和ATP生物荧光法测定血液残留灵敏度的比较研究[J]. 中国预防医学杂志, 2008, 9(8): 708-711 |
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| 作者姓名： | 邢书霞 马玲 王志 张流波 李新武 |
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| 作者单位： | 1. 中国疚病预防控制中心环境与健康相关产品安全所,北京,100021
2. 北京创新世纪生化科技发展有限公司 |
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| 摘 要： | 目的比较蓝光试验、潜血试验和ATP生物荧光法测定血液残留量的灵敏度。方法利用EndocheekTM测试工具箱、杰力试纸和荧光光度计分别测定血液残留量。结果2．5×10^-5的血液稀释液用蓝光试验可100％检出，5×10^-6的血液稀释液用潜血试验可100％检出，ATP生物荧光法可定量测定1．0×10^-9的血液稀释液。结论ATP生物荧光法可比潜血试验和蓝光试验更灵敏地定量测定血液残留量。
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| 关 键 词： | 血液 ATP生物荧光法 潜血试验 蓝光试验 |
Sensitivity of ATP Bioluminescence Assay for Detection of Blood Residue on Medical Instruments Compared with Endo- check Test and Oeeult Blood Test |
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| Affiliation: | XING Shu - xia , MA Ling, WANG Zhi, et al(The Institute for Environment Health and Related Product Safety, China CDC, Beijing 100021, China) |
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| Abstract: | Objective To compare the sensitivity of ATP bioluminescence assay with Endocheck test and occult blood test （OB） for detection of blood residue on medical instrument . Methods Blood residue was determined by using EndocheckTM , lustration test and fluorescence spectrometer. Results Blood dilution of 2.5 × 10^-5 could be detected by Endocheck test, and that of5.0 × 10^-6 could be detected by OB test. ATP bioluminescence assay can detect the blood residue at 1.0 × 10^-9 dilution. Conclusion ATP bioluminescence assay is more sensitive to quantificationally detect blood residue on medical instrument than endocheck test and OB test. |
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| Keywords: | Blood ATP bioluminescence assay Occult blood test Endocheck test |
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