| 分泌表达rhOPG-HSA融合蛋白的毕赤酵母菌株的构建 |
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| 引用本文： | 刘俊丽,张建中,路浩军,刘波,司少艳,施生根,吴军,马清钧. 分泌表达rhOPG-HSA融合蛋白的毕赤酵母菌株的构建[J]. 军事医学科学院院刊, 2009, 33(4): 318-321 |
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| 作者姓名： | 刘俊丽 张建中 路浩军 刘波 司少艳 施生根 吴军 马清钧 |
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| 作者单位： | 1. 解放军第306医院,北京,100101
2. 军事医学科学院生物工程研究所,北京,100850 |
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| 基金项目： | 306医院院级课题资助项目，总后司令部及卫生局课题资助项目 |
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| 摘 要： | 目的：将骨保护素（osteoprotegerin,OPG）与人血清白蛋白（human serum albumin,HSA）基因融合,以构建高效分泌表达的巴斯德毕赤酵母菌株。方法：通过RT-PCR扩增OPG基因,构建pHILD2-HSA-OPG质粒,并对HSA-OPG分泌表达产物进行了ELISA检测。结果：pHILD2-HSA-OPG表达质粒经鉴定与测序证明正确,并在毕赤酵母GS115中能够实现较高表达。结论：成功构建了高效分泌表达重组骨保护素融合蛋白（rhOPG-HSA）的毕赤酵母菌株,为进一步研究其生物学活性奠定了基础。
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| 关 键 词： | 重组骨保护素 血清白蛋白 基因表达 |
Construction of Pichia pastoris yeast strain with high-efficient expression of HSA-OPG |
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| LIU Jun-Li,ZHANG Jian-Zhong,LU Hao-Jun,LIU Bo,SI Shao-Yan,SHI Sheng-Gen,WU Jun,MA Qing-Jun. Construction of Pichia pastoris yeast strain with high-efficient expression of HSA-OPG[J]. Bulletin of the Academy of Military Medical Sciences, 2009, 33(4): 318-321 |
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| Authors: | LIU Jun-Li ZHANG Jian-Zhong LU Hao-Jun LIU Bo SI Shao-Yan SHI Sheng-Gen WU Jun MA Qing-Jun |
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| Affiliation: | LIU Jun-Li , ZHANG Jian-Zhong ,LU Hao-Jun ,LIU Bo, SI Shao-Yart1, SHI Sheng-Gen , WU Jun, MA Qing-Jun(1. The 306th Hospital of PLA, Beijing 100101, China; 2. Institute of Biotechnology, Academy of Military Medical Sci- ences, Beijing 100850, China) |
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| Abstract: | Objective ：To fuse the gene of osteoprotegerin （OPG） with that of human serum albumin （HSA） in order to construct a Pichia pastoris yeast strain with high-efficient expression of HSA-OPG. Methods：The gene of OPG was amplified by RT-PCR and the plasmid of pHILD2-HSA-OPG was constructed. HSA-OPG fusion protein was detected with ELISA kit. Results ：The expression plasmid of pHIL-D2-HSA-OPG was proved to be correct after plamid identification and sequencing, and rhHSA-OPG was relatively efficiently expressed in Pichia pastoris. Conclusion ：Pichi pastoris yeast strain with high-ef- ficient expression of HSA-OPG is successfully constructed, providing a basis for further study of its bioactivity. |
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| Keywords: | rhOPG serum albumin gene expression |
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