|
|||||
|
|
| 气电纺纳米羟基磷灰石-聚羟基丁酸酯复合纤维支架与大鼠成骨细胞体外生物相容性研究 | |
| 引用本文： | 管东华,林映荷,黄春鹏,陈治清. 气电纺纳米羟基磷灰石-聚羟基丁酸酯复合纤维支架与大鼠成骨细胞体外生物相容性研究[J]. 广东牙病防治, 2010, 18(2): 67-71 |
| 作者姓名： | 管东华 林映荷 黄春鹏 陈治清 |
| 作者单位： | 1. 广东省口腔医院·南方医科大学附属口腔医院修复科,广东,广州,510280 2. 四川大学华西口腔医院修复科 3. 海南口腔医院 4. 四川大学华西口腔医学院材料学教研室 |
| 基金项目： | 广东省医学科学技术研究基金(B2009029);;四川省2008年科技计划项目(2008SG0020,2008JY0024-2) |
| 摘 要： | 目的研究气电纺纳米羟基磷灰石-聚羟基丁酸酯[nanosized hydroxyapatite—poly（3-hydroxybutyrate），nHAP-PHB]复合纤维支架对大鼠成骨细胞体外粘附、增殖及骨钙素分泌活性的影响。方法实验组将大鼠成骨细胞接种于气电纺nHAP—PHB复合纤维支架进行体外培养，对照组将大鼠成骨细胞接种于气电纺纯聚羟基丁酸酯（PHB）纤维支架行体外培养。2组进行扫描电镜观察、四甲基偶氮唑盐比色检测及骨钙素检测，评价nHAP—PHB复合纤维支架对大鼠成骨细胞体外粘附、增殖以及骨钙素合成分泌活性的影响。结果扫描电镜观察结果显示接种4h后，与纯PHB纤维支架相比，细胞在nHAP—PHB复合纤维支架上的铺展更完全。培养后第3、7、10、14天，实验组细胞增殖活性均强于对照组（P〈0．05），骨钙素分泌活性均高于对照组（P〈0．05）。结论nHAP—PHB复合纤维中nHAP增强了支架材料与大鼠成骨细胞的生物相容性。 |
| 关 键 词： | 骨组织工程 生物相容性 纳米羟基磷灰石 气电纺聚羟基丁酸酯 |
Biologic Evaluation of a Novel Gas-jet/electrospun Bone Tissue Engineering Scaffolds in vitro |
|
| GUAN Dong-hua,LIN Ying-he,HUANG Chun-peng,et al.. Biologic Evaluation of a Novel Gas-jet/electrospun Bone Tissue Engineering Scaffolds in vitro[J]. Journal of Dental Prevention and Treatment, 2010, 18(2): 67-71 | |
| Authors: | GUAN Dong-hua LIN Ying-he HUANG Chun-peng et al. |
| Affiliation: | GUAN Dong-hua1,LIN Ying-he,HUANG Chun-peng,et al.1Guangdong Provincial Stomatological Hospital & the Affiliated Stomatological Hospital of Southern Medical University,Guangzhou 510280,China |
| Abstract: | Objective To evaluate the biocompatibility of gas-jet/electrospun nanosized hydroxyapatite(nHAP)/poly(3-hydroxybutyrate)(PHB)scaffolds for being used as bone tissue engineering scaffolds.Methods The attachment,proliferation and osteocalcin(OC)secretion of rat osteoblasts cultured on the nHAP-PHB scaffolds were investigated and compared with those cultured on the pure PHB scaffolds.Results PHB-based fibrous scaffolds present good affinity to osteoblasts.Osteoblasts cultured on nHAP-PHB scaffolds presented be... |
| Keywords: | Bone tissue engineering Biocompatibility Nano-hydroxyapatite Gas-jet/electrospun poly （ 3- hydroxybutyrate） |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |