Specific triiodothyronine binding by tumor cells and spleen cells in a thyroid hormone dependent mouse tumor system |
| |
| Affiliation: | 1. Department of Critical Care Medicine, Affiliated Jinling Hospital, Medical School of Southeast University, No. 305 Zhongshan East Road, Nanjing, 210002 Jiangsu, China;2. Department of Critical Care Medicine, Affiliated Jinling Hospital, Medical School of Nanjing University, No. 305 Zhongshan East Road, Nanjing, 210002 Jiangsu, China;3. Pancreatic Center, Department of Gastroenterology, The Affiliated Hospital of Yangzhou University, Yangzhou University, No. 368 Hanjiang Media Road, Yangzhou, 225000 Jiangsu, China;4. Yangzhou Key Laboratory of Pancreatic Disease, Institute of Digestive Diseases, The Affiliated Hospital of Yangzhou University, Yangzhou University, No. 368 Hanjiang Media Road, Yangzhou, 225000 Jiangsu, China;1. Department of Thyroid and Neck Tumor, Tianjin Medical University Cancer Institute and Hospital, National Clinical Research Center for Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin’s Clinical Research Center for Cancer, Tianjin 300060, China;2. School of Science, Technology and Engineering, University of the Sunshine Coast, Maroochydore DC, Queensland 4558, Australia;3. State Key Laboratory of Medicinal Chemical Biology, College of Life Sciences, Nankai University, 300071 Tianjin, China;4. School of Medicine, Nankai University, Tianjin, China;5. Department of Thyroid and Breast Surgery, Tianjin Key Laboratory of General Surgery in Construction, Tianjin Union Medical Center, Tianjin, China;1. Department of Oncology, The Third Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453000, PR China;2. Xinxiang Engineering Technology Research Center of Immune Checkpoint Drug for Liver-Intestinal Tumors, Xinxiang Medical University, Xinxiang, Henan 453000, PR China;3. Department of Pathology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453000, PR China;4. Department of Immunology, School of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan 453000, PR China;5. Henan Key Laboratory of Medical Tissue Regeneration, College of Basic Medical Sciences, Xinxiang Medical University, Xinxiang, Henan, PR China;6. Henan International Joint Laboratory of Immunity and Targeted Therapy for Liver-Intestinal Tumors, Xinxiang Medical University, Xinxiang, Henan 453000, PR China |
| |
| Abstract: | We have reported thyroid hormone dependency of syngeneic mouse tumor (fibrosarcoma T241 in C57Bl/6J mice) for growth and spread. To study further the mechanisms of thyroid hormone action we assayed tumor cells and spleen cells for thyroid hormone receptors. Cells were incubated at 37°C for 1 hr with [125I]triiodothyronine with and without unlabeled T3. Competitive inhibition of [125I]T3 bound to spleen cells was demonstrated by increasing the amount of unlabeled T3 and T4. Similar competitive inhibition of binding was minimal for intact tumor cells. Saturable binding sites in muclei were demonstrated in all these cell populations. A Scatchard analysis of nuclei fraction from these cells showed equilibrium dissociation constant (Kd) of 0.56 × 10−9 for normal spleen cells and tumor cells and 1.4 × 10−9 M for tumor-bearing mice spleen cells. The estimated maximum binding capacity for nuclei of these cells was 26 fmol/10 × 106 cells for normal spleen cells, 46 mol/10 × 106 cells for tumor-bearing mice spleen cells and 45 fmol/10 #x0000D7; 106 cells for tumor cells. Our results established the presence of T3 receptors in nuclei of tumor cells as well as spleen cells and suggests the direct metabolic effect of thyroid hormone as a possible mechanism for thyroid hormone dependency of this tumor. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
