痛风康宁片治疗痛风性关节炎的药效学研究

目的 采用多种实验模型评价痛风康宁片防治痛风性关节炎的有效性。方法 采用微晶型尿酸钠（MSU）诱导大鼠急、慢性痛风性关节炎模型，观察痛风康宁片对急、慢性痛风性关节炎的预防和治疗作用；采用大鼠棉球肉芽肿模型，观察痛风康宁片的抗炎作用；采用血瘀大鼠模型，观察痛风康宁片的活血作用；采用小鼠醋酸扭体模型，观察痛风康宁片的镇痛作用。结果 与模型组比较，痛风康宁片预防给药，1.15、2.3 g/kg能明显减轻MSU引起的大鼠右踝关节肿胀、改善步态、减少血清中白细胞介素（IL）-1β、IL-6含量；痛风康宁片治疗给药，1.15、2.3 g/kg能明显减轻MSU引起的大鼠右踝关节肿胀、改善步态；痛风康宁片1.15、2.3 g/kg明显降低慢性关节炎大鼠气囊灌洗液中IL-1β、IL-6、肿瘤坏死因子-α（TNF-α）含量和吞噬细胞数；痛风康宁片1.15、2.3 g/kg能明显减轻大鼠棉球肉芽重量；痛风康宁片0.58、1.15、2.3 g/kg明显抑制血瘀大鼠全血粘度的升高；痛风康宁片1.66、3.33 g/kg组明显减少醋酸诱导的小鼠扭体次数。结论 痛风康宁片有明显的抗炎、镇痛及活血作用，对痛风性关节炎有明显的治疗作用。

Pharmacodynamic study of Tongfeng Kangning Tables in treatment of gouty arthritis

Objective To evaluate the effectiveness of Tongfeng Kangning Tables in prophylactic and therapeutic of gouty arthritis in various experimental models. Methods To observe the preventive and therapeutic effects of Tongfeng Kangning Tables on acute and chronic gouty arthritis in rats induced by microcrystalline sodium urate (MSU). The anti-inflammation effect of Tongfeng Kangning Tables was observed by using cotton ball granuloma model in rats. Blood stasis rat model was used to observe the blood-activating effect of Tongfeng Kangning Tables. The analgesic effect of gout Tongfeng Kangning Tables was observed by using acetic acid writhing model in mice. Results Compared with model group, prophylactic administration of Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the swelling of right ankle joint induced by MSU, improve gait, and reduce the contents of IL-1β and IL-6 in serum. Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the swelling of the right ankle and improve the gait induced by MSU. The contents of IL-1β, IL-6, tumor necrosis factor-α (TNF-α) and the number of phagocytic cells in the balloon lavage fluid of chronic arthritis rats were significantly reduced in the 1.15 and 2.3 g/kg Tongfeng Kangning Tables groups. Tongfeng Kangning Tables in 1.15 and 2.3 g/kg groups could significantly reduce the weight of cotton ball granulation. Tongfeng Kangning Tables in 0.58, 1.15, and 2.3 g/kg groups significantly inhibited the increase of whole blood viscosity of blood stasis rats. Tongfeng Kangning Tables 1.66 and 3.33 g/kg groups significantly reduced the number of body twisting induced by acetic acid. Conclusion Tongfeng Kangning Tables has obvious anti-inflammatory, analgesic and blood-activating effects, and has obvious therapeutic effects on gouty arthritis.