Comparison of 111In-oxine and 111In-Acetylacetone for the labeling of cells: in vivo and in vitro biological testing |
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| Affiliation: | 1. Institute of Agricultural Bio-Environmental Engineering, College of Bio-Systems Engineering and Food Science, Yuhangtang Road 866, Hangzhou 310058, PR China;2. Swedish Centre for Resource Recovery, University of Borås, Borås 50190, Sweden;3. The Rural Development Academy, Zhejiang University, Yuhangtang Road 866, Hangzhou 310058, PR China;4. Aquaculture and Fisheries Group, Department of Animal Sciences, Wageningen University, 6708 WD Wageningen, The Netherlands;5. Institute of Bioresource Engineering, Nanjing Technology University, Nanjing 210009, PR China;6. Bioresource Institute for Healthy Utilization (BIHU), Zunyi Medical University, Zunyi, Guizhou 563000, China;7. Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China;1. Key Lab of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China;2. University of Chinese Academy of Sciences, Beijing 100049, China |
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| Abstract: | We have compared several complexes of indium as cell labels: indium-111-acetylacetone, indium-111-oxine, and indium-111-chloride complexed with 8-hydroxyquinoline (oxine) immediately prior to use. In labelling with acetylacetone, it was shown that the labeling efficiency is directly proportional to the amount of acetylacetone present, but the cell viability (as measured by in vitro aggregation studies), is inversely proportional to the amount of acetylacetone present. Biological studies were carried out in dogs using indium-111-labeled platelets; survival times and recovery values obtained with platelets labeled using all three techniques were similar. The same solutions were also used to label white blood cells; labeling efficiencies of greater than 80% were obtained in all cases, and the viability (as measured by trypan blue exclusion) was high in all cases. Chemotactic ability of the white cells labeled with indium-111-oxine is higher than that of unlabeled control cells; however, cells labeled with indium-111-acetylacetone were the same as the unlabeled control cells. |
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