| Abstract: | Prekallikrein, plasminogen, and prothrombin of human blood plasma are separately activated by kaolin, streptokinase, or thromboplastin. Measurement of the TAME-esterase (TAME: N -tosyl-L-arginine methyl ester) activity of each of the enzymes thus produced and its changes during incubation of the plasma with the activator can be used to estimate the content of kallikrein, plasmin, and thrombin and their inhibitors. Evidence is given that under the conditions of determination as described activation is specific for each enzyme and does not affect the level of the other precursors. The method is developed in modifications enabling the values of seven indices to be obtained with 0.4–0.7 ml of blood plasma.Insitute of General Pathology and Pathophysiology, Academy of Medical Sciences of the USSR, Moscow. (Presented by Academician of the Academy of Medical Sciences of the USSR A. M. Chernukh.) Translated from Byulleten' Éksperimental'noi Biologii i Meditsiny, Vol. 81, No. 5, pp. 632–634, May, 1976. |
