混配农药对家兔诱发电位及脑组织ATP酶的影响

目的研究混配农药对家兔中枢神经系统功能的影响及其机制。方法将家兔随机分为混配农药染毒A组（丙溴磷与氰戊菊酯混配染毒组）、混配农药染毒B组（丙溴磷与灭多威？昆配染毒组）和1个对照组，每组6只。3个染毒组分别给予3种染毒剂量进行染毒，于染毒前后进行家兔体感染诱发电位（SEP）及染毒后大脑组织三磷酸腺苷（ATP）酶活力的测定。结果与对照组比较，2个混配农药染毒组的高剂量组染毒后SEP的N1、P1、N2波潜伏时均显著延长（P〈0．01），2个混配农药染毒组的高中剂量组染毒后的Na^＋-K^＋-ATP酶、Ca^2＋-Mg^2＋-ATP酶的活力明显下降（P〈0．05。P〈0．01）。结论一定剂量的混配农药可导致家兔中枢神经系统功能的的改变，这种改变可能与大脑组织中N^＋-K^＋-ATP酶、Ca^2＋-Mg^2^＋-ATP酶活力的下降有关．

Effects of mixed pesticides on somatosensory evoked potential and activities of ATPase of brains in rabbits

Objective To explore the effects of mixed pesticides on the Somatosensory evoked potential(SEP)and the activities of ATPase of brains in rabbits. Methods Rabbits were divided into 2 mixed pesticide exposure groups and 1 control group.A group were poisoned by profenofos and fenvalerate,and B group were poisoned by profenofos and methomyl.The SEP were measured in each group before and after poisoning by mixed pesticide,and the activites of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase of the brain was measured in each group after poisoning by mixed pesticide. Results Compared with the control group,the potential periods of N1,P2,N2 in high-dose exposure group in A group and B group delayed significantly after poisoning by mixed pesticide(P<0.01).Compared with the control group,the activities of Na~+-K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase decreased significantly in high-dose exposure groups and middle-dose groups in A group and Bgroup after poisonging by mixed pesticide(P<0.05,P<0.01). Conclusion The mixed pesticides can harm the functions of central nervous system,which must be related to the decrease of the activities of Na~+K~+-ATPase and Ca~(2+)-Mg~(2+)-ATPase in brain.