Purification and characterization of Moran 20K fromMorus alba |
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Authors: | Eun-Sun Kim Sung-Jean Park Eun-Ju Lee Bak-Kwang Kim Hoon Huh Bong-Jin Lee |
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Institution: | (1) Department of Pharmacy, Seoul National University, 151-742 Seoul, Korea |
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Abstract: | A new glycoprotein was purified from the aqueous methanolic extract of the root bark ofMorus alba which has been used as a component of antidiabetic remedy in Oriental Medicine. SDS-PAGE result shows that the molecular
weight of the glycoprotein was approximately 20 kDa. This new glycoprotein was named as Moran 20K. The protein lowered blood
glucose level in streptozotocin-induced hyperglycemic mice model and it also increased the glucose transport in cultured epididymis
fat cells. The amino acid composition of the protein was analyzed, and the protein contained above 20% serine and cysteine
such as insulin. The actual molecular weight of the protein was determined as 21,858 Da by MALDI-TOF mass spectroscopy. |
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Keywords: | Moran 20K Glycoprotein MALDI-TOF Mass |
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