胰酪胨大豆培养基和改良马丁培养基的微生物促生长能力考察

目的：考察国产胰酪胨大豆培养基（TSB）和改良马丁培养基（MMB）对微生物的促生长能力,为〈中国药典〉无菌检查法培养基的选择提供依据.方法：采用培养基灵敏度检查法和菌数平皿计数法,以BD公司生产的TSB为对照,对微生物的促生长情况进行考察.结果：单因素方差分析结果显示,所用国产TSB和MMB培养基在两种方法中对代表性微生物促生长能力无显著性差异.结论：〈中国药典〉无菌检查用培养基应与国际先进国家药典保持一致,采用TSB替代MMB培养基用于需气菌和真菌的培养.

Microbial Growth Promotion Evaluation of Tryptic Soybean Medium and Modified Matin Medium

Objective： To evaluate microbial growth promotion of tryptic soybean medium （TSB） and modified matin medium （MMB） from different manufactures in China. Methods： Sensitivity test method and plate - count method were applied using six microorgan-isms from different genus, respectively. TSB and MMB from three domestic manufactures in China were compared, and TSB from BD company was set as control group. Results： According to one - way analysis of variance, there was no significant difference between TSB and MMB media of growth promotion using both methods in general. Conclusions： Complying with the media system of pharmaco-poeia in developed countries, TSB can be applied in sterility test of Chinese Pharmacopoeia instead of MMB, if the quality of media could be continually stable.