结肠癌组织游离氨基酸与肿瘤体积相关性研究

目的：探讨结肠癌生长对氨基酸代谢的影响以及对某些氨基酸的特殊需求。方法：采用反相高压液相色谱技术测定18例结肠癌病人肿瘤组织20种游离氨基酸含量，与肿瘤体积进行相关性和多元逐步回归分析。结果：结肠癌组织中谷氨酰胺、蛋氨酸、精氨酸、苏氨酸、缬氨酸、亮氨酸、苯丙氨酸、天冬酰胺、丝氨酸含量较正常结肠粘膜组织明显增加（P＜0．05），而丙氨酸、色氨酸含量较正常结肠粘膜组织明显减少（P＜0．05）。结肠癌组织游离谷氨酰胺、蛋氨酸、精氨酸、缬氨酸、亮氨酸、天冬酰胺、丝氨酸与肿瘤体积呈明显正相关。结论：随着结肠癌生长，肿瘤特别需求谷氨酰胺、蛋氨酸、精氨酸、缬氨酸、亮氨酸、天冬酰胺、丝氨酸，这为结肠癌病人氨酸酸营养液的组成提供了参考资料。

The study on interrelation between colonic carcinoma tissue free amino acid and tumor volume

Objectives:In order to observe the effect of colonic carcinoma growth on amino acid metabolism. Methods:Levels of tumor tissue 20 free amino acids were measured from 18 patients with colonic carcinoma, and the normal colonic mucosa was used as controls.Reversed Phase High Performance Liquid Chromatography was used for the determination.The correlations between tumor volume and tumor tissue 20 free amino acids were analysed. Results:The contents of colonic carcinoma tissue free glutamine,methionine,arginine,threonine,valine,leucine,phenylalanine,aspargine and serine were significantly increased and the contents of tumor tissue free alanine and tryptophan were significantly decreased when compared with those of normal colon mucosa( P <0.05).A positive correlation was found between tumor volume and tumor tissue free glutamine,methonine,arginine,valine,leucine,asparagines or serine. Conclusions:A large number of amino acids are found to be increased in the tissue of colonic carcinoma,such as glutamine,methionine,argnine,valine,leucine,asparagines,serine.