ELISA法测定谷物中伏马毒素

为测定谷物中伏马毒素含量，了解粮食中有关真菌的污染状态，评价伏马毒素的ＥＬＩＳＡ测定法。采集市售大麦、玉米及饲料玉米，采用酶联免疫吸附法测定，对阳性样品进行真菌培养分离，并用ＨＰＬＣ进行确证。结果：４０份大米中未检出伏马毒素，７０份玉米检出阳性样品２７份，均数０．６８ｍｇ／ｋｇ。ＨＰＬＣ法的确证结果表明２７份ＥＬＩＳＡ法阳性样品中２３份检出伏马毒素Ｂ１，均数为０．４２ｍｇ／ｋｇ；６份同时检出伏马毒素Ｂ２，均数为０．２ｍｇ／ｋｇ。２７份阳性样品真菌分离培养结果均检出串珠镰刀菌。结论：玉米受串珠镰刀菌污染较重，并可有伏马毒素产生。本次调查大米中未检出伏马毒素，情况较好。其它主粮中伏马毒素污染情况还有待进一步调查

Determination of Fumonisins in Cerals by ELISA

おo determinate fumonisin B_1 in cereals, to learn the extent of fungal contamination in cereals, and to evaluate the method of ELISA in the determination of fumonisions. Method:The Samples were collected from market and determined by ELISA kit. The positive ones were confirmed by mycological separation and culture, and determined by HPLC. Result: No fumonisins was detected in 40 rice samples, but 27 out of 70 corn samples were positive with all average concentration of 068mg/kg. The positive samples were also determined by HPLC, and 23 were formed to contain fumonisin B1 (average 042mg/kg) in which 6 sample were found fumonisin B2 (average 02mg/kg). In all the positive samples, F. moniliforme was identified by culture. Conclusion: Corn was easily contaminated by F.moniliforme which produced fumonisins. Although all rice samples were negative, further investigation is need to determine fumonisins in other cereals.