分散固相萃取-气相色谱串联质谱法测定菊花茶中的56种农药残留

目的 建立菊花茶中56种农药残留的分散固相萃取（QuEChERS） - 气相色谱串联三重四级杆质谱（GC - MS/MS）测定方法。方法 样品用乙腈提取，PSA+C18+GCB混合型分散固相萃取剂净化，DB - 5MS色谱柱分离，多反应监测模式（MRM）检测，外标法定量。结果 在0.005～500 mg/L范围内，56种农药的线性相关系数均≥0.995，方法定量限为0.002～0.057 mg/kg，平均回收率为60.0%～118.0%，相对标准偏差（RSD）为0.53%～20.09%。结论 该方法简单快速灵敏，可以实现菊花茶中农药残留的快速高通量检测。

Determination of 56 pesticide residues in chrysanthemum tea by dispersive solid-phase extraction and GC-MS/MS

Abstract: Objective To establish a method for the determination of 56 pesticide residues in chrysanthemum tea by dispersive solid-phase extraction and chromatography-gas chromatography-mass spectrometry (GC-MS/MS). Methods The pesticides in the samples were extracted with acetonitrile and cleaned up by solid-phase dispersion of PSA+C18+GCB. The pesticides in the extracts were separated with DB-5MS column and detected by MS/MS with multi-reaction monitoring (MRM) mode. The targets were quantified with the external standard method. Results The method showed good linearity (r≥99.5) in the range of0.005-500mg/L. The limits of quantification (LOQs) were 0.002 -0.057mg/kg. The recoveries of the method were 60.0% ~118.0% , with the relative standard deviations (RSDs) of 0.53％ -20.09％ . Conclusion The method is simple, rapid, and sensitive. It can be used for the high throughput rapid screening for pesticide residues in chrysanthemum tea.