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蒙药三红汤中大叶茜草素的含量测定
引用本文:锡林通嘎拉嘎,水兰,包勒朝鲁. 蒙药三红汤中大叶茜草素的含量测定[J]. 现代中医药, 2021, 0(2): 35-39
作者姓名:锡林通嘎拉嘎  水兰  包勒朝鲁
作者单位:内蒙古自治区人民医院;内蒙古医科大学
基金项目:内蒙古自治区科技计划项目-鹿角等20种蒙药材一般炮制工艺及质量标准研究。
摘    要:目的 建立高效液相色谱法,测定蒙药三红汤中大叶茜草素的含量.方法 大叶茜草素含量测定色谱柱为KromilC18(250mm×4.6mm,5μm),流动相甲醇-水-四氢呋喃(77:22:1),检测波长为390 nm,流速为1.0 ml/min,柱温35℃.结果 大叶茜草素在166.64μg~2499.6μg(r=0.99...

关 键 词:蒙药  三红汤  大叶茜草素  含量测定

Determination of Rubicin in Mongolian Medicine Sanhong Decoction
Affiliation:(People's hospital of Inner Mongolia Autonomous Region,Hohhot 010017,China;Inner Mongolia medical university,Hohhot 010010,China)
Abstract:Objective To establish an HPLC method for the determination of Rubicin in Mongolian medicine Sanhong decoction.Methods The chromatographic column was kromil C18(250 mm×4.6 mm,5μm),the mobile phase was methanol-water-tetrahydrofuran(77:22:1),the detection wavelength was 390 nm,the flow rate was 1.0 ml/min,and the column temperature was 35℃.Results The linear range of Rubicin was 166.64μg~2499.6μg(r=0.9999).The average recovery was 100.34%.Conclusion The method is simple,sensitive,accurate and reproducible,and can be used for the determination of Rubicin in Mongolian medicine Sanhong decoction.
Keywords:Mongolian medicine  Sanhong Decoction  Rubicin  Content determination
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