肌醇六磷酸对溃疡性结肠炎大鼠保护作用的实验研究

目的 研究肌醇六磷酸（IP6）对溃疡性结肠炎大鼠的保护作用，并初步探讨其作用机制。方法 48只雄性SD大鼠随机分为正常组、模型组、柳氮磺吡啶组、IP6低、中、高剂量组，采用葡聚糖硫酸钠诱导法构建溃疡性结肠炎大鼠模型，相应组分别以肌醇六磷酸钠0.5g/kg bw、1.0g/kg bw和2.0g/kg bw，柳氮磺吡啶0.5g/kg bw灌胃，连续10天。评估疾病活动指数（DAI）和结肠组织宏观损伤评分，结肠组织病理学观察，并检测结肠组织超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX）的活力，丙二醛（MDA）、肿瘤坏死因子α（TNF-α）和白细胞介素1β（IL-1β）的含量。结果 与模型组比较，柳氮磺吡啶组和IP6低、中、高剂量组DAI、结肠宏观损伤评分降低，差异有统计学意义（F=33.637，P<0.001；F=33.369，P<0.001），组织病理学观察溃疡性结肠炎明显改善，且结肠组织中MDA、TNF-α和IL-1β含量均降低，差异有统计学意义（F=85.892，P<0.001；F=359.605，P<0.001；F=66.698，P<0.001），IP6高剂量组SOD活力和GSH-Px活力最高，差异有统计学意义（F=7.073，P=0.001；F=40.812，P<0.001），且随IP6剂量增加而升高。结论 IP6明显改善溃疡性结肠炎大鼠肠道病变，与IP6可抑制促炎因子，增强抗氧化作用有关。

Protective effect of Inositol hexaphosphate on ulcerative colitis in rats

Objective To explore the protective effect and its mechanism of inositol hexaphosphate(IP6) in ulcerative colitis rat.Methods 48 male SD rats were randomly divided into normal group, model group, salazosulfopyridine group, low dose IP6 group, medium dose IP6 group and high dose IP6 group. An experimental rat model of ulcerative colitis was induced by dextran sodium sulfate. Inositol hexaphosphate sodium 0.5 g/kg BW, 1.0 g/kg BW, 2.0 g/kg BW, and salazosulfapyridine sodium 0.5 g/kg BW were administered to the corresponding group for 10 consecutive days. The effect was evaluated through appraising the disease activity index( DAI),colon macro injury score and histopathology observation. The activity of SOD and GSH-Px in colon tissues were measured, and the contents of MDA, tumor necrosis factor α(TNF-α) and interleukin1β(IL-1β) in colon tissues were determined. Results Compared with the model group, DAI and colon macro injury scores were decreased in the salazosulfapyridine group and each IP6 dose groups, and the difference was statistically significant(F=33.637,P<0.001;F=33.369,P<0.001), the ulcerative colitis was significantly improved by histopathological observation, and the contents of MDA, TNF-α and IL-1β in colonic tissues were decreased, and the difference was statistically significant(F=85.892,P<0.001;F=359.605,P<0.001,F=66.698,P<0.001). SOD activity and GSH-Px activity increased in each IP6 does group,and the difference was statistically significant(F=7.073,P=0.001,F=40.812,P<0.001). Conclusion IP6 significantly improved intestinal lesions in ulcerative colitis rats by inhibiting the production of pro-inflammatory factors and enhancing the antioxidant effect.