三七不同药用部位中人参皂苷Rg3的含量测定

目的 建立三七中20(S)-人参皂苷Rg3和20(R)-人参皂苷Rg3的含量测定方法,评价不同药用部位及加工方式的三七提取物中,两个型的人参皂苷Rg3的含量情况.方法 采用高效液相色谱法测定,色谱条件:ECOSIL 120-5-C18-SH色谱柱(4.6×250mm,5μm);流动相为乙腈-异丙醇-水(47:3:50)...

Determination of Ginsenoside Rg3 in Different Parts of Panax Notoginseng

Objective To establish a method for the determination of 20(s)-Ginsenoside Rg3 and 20(R)-Ginsenoside Rg3 in Panax notoginseng,and to evaluate the content of two types of Ginsenoside Rg3 in Panax notoginseng extracts from different medicinal parts and processing methods.Methods HPLC was used to determine the content.The chromatographic conditions were as follows:ecosil 120-5-c18-sh column(4.6×250mm,5μm);The mobile phase was acetonitrile-isopropanol-water(47:3:50);the flow rate was 1.0ml·min^-1;the detection wavelength was 203nm;the injection volume was 10μL;the column temperature was 20℃;the contents of 20(s)-Ginsenoside Rg3 and 20(R)-Ginsenoside Rg3 in different medicinal parts and processing methods of Panax notoginseng extract were determined.Results the linear range of 20(s)-Ginsenoside Rg3 was 14.0~279.6μg·ml^-1(r²=1.0000).The average recovery was 100.56%,RSD 2.46%.The linear range of 20(R)-Ginsenoside Rg3 was 14.1~282.0μg·ml^-1(R²=0.9999).The average recovery was 101.51%,RSD 2.99%.Conclusion The content of 20(s)-Ginsenoside Rg3 in cooked Panax notoginseng increased significantly.