循环miRNA-210的表达与胎儿生长受限的相关性分析

目的 分析miRNA-210与胎儿生长受限的相关性,阐明循环中miRNA-210在胎儿生长受限发病机制中的作用,为胎儿生长受限的早期诊断及病理机制提供新的生物学标志物.方法 选择2015年1月至2016年1月在扬州市妇幼保健院分娩的产妇,采取随机原则,选取符合胎儿生长受限孕足月分娩(包括顺产及剖宫产)的30例孕妇为胎儿生长受限组,选取同期足月分娩正常体重新生儿的孕妇30例为对照组,采集两组孕妇外周血,提取RNA采用Trizol法,Real Time-PCR分别测定胎儿生长受限组和对照组循环中miRNA-210的表达水平与选择内参基因U6表达的相对值,分析miRNA-210与胎儿生长受限的相关性.结果 胎儿生长受限组与对照组产妇年龄、孕前体重、孕周比较无明显差异(t值分别为0.84、0.74、0.48,均P>0.05),但胎儿生长受限组新生儿体重明显低于对照组,差异有统计学意义(t=8.56,P<0.01).胎儿生长受限组miRNA-210的表达水平为4.58,与对照组相比,表达明显增加(t=7.43,P<0.01).miRNA-210可作为一个潜在的生物学标志物来区分胎儿生长受限及正常发育儿.结论 胎儿生长受限组循环中miRNA-210的表达水平明显升高,miRNA-210可能参与了胎儿生长受限的发病,循环中miRNA-210可作为胎儿生长受限诊断的生物标志物.

Correlation analysis of circulating miRNA-210 expression level with fetal growth restriction

Objective To study the relationship between miRNA-210 and fetal growth restriction(FGR)and clarify the effect of circulating miRNA-210 in the pathogenesis of FGR,so as to provide new biomolecular maker for early diagnosis and pathogenesis of FGR.Methods From January 2015 to January 2016 random principle was adopted to select 30 prenant women delivering full-term neonates with FGR in FGR group and 30 cases of normal controls in control group.RNA was extracted and Trizol reagent and Real Time-PCR were used to determine circulating miRNA-210 expression level and relative value of reference gene U 6 to analyze the correlation of miRNA-210 with FGR.Results There was no obvious difference between FGR group and normal control group in maternal age,pre-pregnancy weight and gestational age(t value was 0.84,0.74 and 0.48,respectively,all P>0.05),but neonatal weight of FGR group was remarkably lower than that of the control group with statistical difference(t=8.56,P<0.01).The level of circulating miRNA-210 was 4.58 in FGR group,which increased significantly compared to the control group(t= 7.43,P<0.01).MiRNA-210 might be used as a potential biological marker to distinguish neonates with FGR from normal ones.Conclusion The expression level of miRNA-210 is significantly increased in FGR group,and miRNA-210 may be involved in the pathogenesis of FGR.Circulating miRNA-210 can be used as biomarker in early diagnosis of FGR.