| 新型酪氨酸激酶抑制剂XCF-43b体外抗血管新生研究 |
| |
| 引用本文： | 暴亚锋,刘飞飞,刘静,张继虹. 新型酪氨酸激酶抑制剂XCF-43b体外抗血管新生研究[J]. 医学分子生物学杂志, 2017, 14(3). DOI: 10.3870/j.issn.1672-8009.2017.03.002 |
| |
| 作者姓名： | 暴亚锋 刘飞飞 刘静 张继虹 |
| |
| 作者单位： | 昆明理工大学衰老与肿瘤遗传学重点实验室 昆明市,650500 |
| |
| 基金项目： | 国家自然科学基金，云南省自然科学基金，grants from the National Natural Science Foundation of China，the Natural Science Foundation of Yunnan Province |
| |
| 摘 要： | 目的 探究XCF-43b 体外抗血管新生机理.方法 鸡胚尿囊膜(CAM)检测化合物抑制血管新生能力,MTT检测其对HUVEC细胞增殖影响,划痕实验和微管形成实验检测化合物对HUVEC迁移和微管形成的影响,Western印迹检测VEGFR2信号通路相关蛋白表达情况.结果 XCF-43b能够抑制CAM血管新生,抑制HUVEC细胞增殖的IC50为(28.42±7.23) μmol/L,对在VEGF刺激下的HUVEC的IC50值为(9.03±1.28) μmol/L,此外,2.5 μmol/L的XCF-43b能够抑制HUVEC细胞的迁移和微管形成;且能够抑制VEGFR2及其下游信号因子的激活.结论 XCF-43b通过抑制VEGFR2信号通路来抑制血管新生.
|
| 关 键 词： | 抗血管新生 VEGFR2 |
Mechanism of In Vitro Anti-Angiogenesis of Tyrosine Kinase Inhibitor XCF-43b |
| |
| BAO Yafeng,LIU Feifei,LIU Jing,ZHANG Jihong. Mechanism of In Vitro Anti-Angiogenesis of Tyrosine Kinase Inhibitor XCF-43b[J]. Journal of Medical Molecular Biology, 2017, 14(3). DOI: 10.3870/j.issn.1672-8009.2017.03.002 |
| |
| Authors: | BAO Yafeng LIU Feifei LIU Jing ZHANG Jihong |
| |
| Abstract: | Objective To explore the in vitro anti-angiogenesis mechanism of XCF-43b.Methods The proliferation of human umbilical vein endothelial cells (HUVEC) was detected by MTT assay and the migration and tube formation of HUVEC were studied by scratches and tube formation.Western blotting was used to detect the expression of VEGFR2 signaling pathway.Results Our results showed that XCF-43b was able to inhibit angiogenesis by CAM.MTT assay exhibited that XCF-43b could inhibit the proliferation of HUVEC cells.In addition,XCF-43b was found to inhibit the migration of HUVEC and microtubule formation.Western blot analysis revealed that XCF-43b was able to inhibit VEGFR2 signaling pathway.Conclusion XCF-43b inhibits angiogenesis by inhibiting VEGFR2 signaling pathway. |
| |
| Keywords: | tumor anti-angiogenesis VEGFR2 |
| 本文献已被 万方数据 等数据库收录！ |
