用两种PCR方法对HIV-1测定的敏感度对比分析

目的:探讨用Roche公司生产的Combas Amplicor HIV-1病毒载量检测试剂与巢式RT-PCR法检测HIV-1这两种检测方法之间的相关性。方法:将81份HIV-1阳性患者血浆样本首先用Combas Amplicor病毒载量检测试剂盒检测出病毒拷贝数/ml,根据病毒拷贝数对数/ml的多少分为5组。再用巢式RT-PCR法对以上样本进行重复测定,观察以上2种检测方法的相关性。结果:用巢式RT-PCR法对用Combas Ampli-cor病毒载量检测的病毒拷贝数/ml分为2.000~2.999组;3.000~3.999组;4.000~4.999组;5.000~5.999组和>6.000组病毒拷贝数/ml的检测阳性率分别为87.5%(7/8),93.75%(30/32),95.83%(23/24),100%(14/14)和100%(3/3)。结果表明,随着病毒拷贝数/ml的增高巢式法检测的阳性率也相应增高。结论:巢式RT-PCR由于具有操作简单,价格便宜等优点可作为AIDS的初步筛查,指导抗AIDS药物治疗等使用,对于基层而言是一种简便易行的方法。

Sensitive analysis on HIV-1 determined by 2 PCR methods

Objective:To investigate the relativity between quantitative detection of HIV-1 by Combas Amplicor virus load kit produced by Roche Company and qualitative detection of HIV-1 through nested RT-PCR method.Methods:81 HIV-1 blood samples were obtained from HIV areas in Henan,first tested viral load copies/ml with quantitative Combas Amplicor,divided into 5 groups according to viral copies/ml then,re-tested above data by nested RT-PCR,observe the coincidence rate between this 2 Methods.Results:Compas Amplicor viral copies/ml as follows,2.000-2.999 group;3.000-3.999 group;4.000-4.999 group;5.000-5.999 group and above 6.000 group,while each of the number of positive rate were 87.5%(7/8),93.75%(30/32),95.83%(23/24),100%(14/14) and 100%(3/3).The results indicate,with the viral copies/ml become higher,the positive rate will be raise by nested RT-PCR.Conclusion:The teginning of period tested AIDS by nested RT-PCR,can guide therapy of at-AIDS,furthermore,it has advavtage of process simple,price inexpensive,for the basic unit it is a method which can carry out.