高效液相色谱法测定附桂通便颗粒中苯甲酰新乌头原碱和新乌头碱的量

目的对附桂通便颗粒中苯甲酰新乌头原碱、新乌头碱进行含量测定。方法运用高效液相色谱法（HPLC）,Kromasil C18柱（250 mm×4.6 mm,5μm）,流动相为0.015%二乙胺-乙腈溶液,线性梯度洗脱,流速为1.0 mL/min,柱温35℃,检测波长240 nm。结果苯甲酰新乌头原碱、新乌头碱分别在0.088 8-0.888 0μg（r=0.999 8）、0.199 0-1.990 0μg（r=0.999 8）范围内,具有良好的线性关系;平均加样回收率依次为99.11%、99.13%,相对标准偏差（RSD）分别为0.76%、1.61%。结论该方法快速、简便,重现性好,可作为该制剂的定量分析方法。

Content determination of benzoylmesaconitine and mesaconitine in Fugui Tongbian Granules by HPLC

Objective To determine the contents of benzoylmesaconitine and mesaconitine in Fugui Tongbian Granules by HPLC.Methods The high performance liquid chromatographic（HPLC） method was adopted on the Kromasil C18 column（250 mm×4.6 mm,5 μm）,the mobile phase was 0.015% diethylamine-acetonitrile with linear gradient elution.The flow rate was 1.0 mL/min,the column temperature was 35 ℃ and the detection wavelenghth was 240 nm.Results The good linear relationship was 0.088 8-0.888 0 μg（r=0.999 8） for benzoylmesaconitine and 0.199 0-1.990 0 μg formesaconitine（r=0.999 8）,respectively.The average recoveries were 99.11% and 99.13%,RSD 0.76% and 1.61%,respectively.Conclusion This method is rapid and simple with good reproducibility and can be used as a quantitative analysis method of the preparation.