HPLC法同时测定参芪五味子胶囊中五味子酯甲和五味子甲素的含量

目的:建立同时测定参芪五味子胶囊中五味子酯甲和五味子甲素含量的方法。方法:采用高效液相色谱法。色谱柱为Waters Xterra Ms C18,流动相为乙腈-四氢呋喃-水(18∶18∶64,V/V/V),流速为1.0 ml/min,检测波长为222 nm,柱温为25℃,进样量为10μl。结果:五味子酯甲和五味子甲素进样量分别在0.026 2⁰.418 4、0.024 90.418 4、0.024 9⁰.398 4μg范围内与各自峰面积积分值呈良好的线性关系(均r=0.999 9);精密度、稳定性、重复性试验的RSD≤1.7%;平均加样回收率分别为101.1%、100.8%,RSD分别为1.6%、1.5%(n=9)。结论:该方法重复性好、灵敏度高、结果准确可靠,可用于参芪五味子胶囊的质量控制。

Simultaneous Determination of Schisantherin and Deoxyschizandrin in Shenqi Wuweizi Capsule by HPLC

OBJECTIVE： To establish a method for the determination of schisantherin and deoxyschizandrin in Shenqi wuweizi capsule. METHODS： The separation was performed on Waters Xterra Ms C18 column with acetonitrile-tetrahydrofuran-water （18： 18：64, V/V/V） as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was set at 222 nm, and the column temperature was 25 ℃. The injection volume was 10 ul. RESULTS： The linear range of schisantherin and deoxyschizandrin were 0.026 2-0.418 4 and 0.024 9-0.398 4 ug （r=0.999 9） ; RSDs of precision, stability and reproducibility tests were all lower than 1.7% ; the average recoveries were 101.1% （RSD=1.6%, n=9） and 100.8% （RSD=1.5%, n=9）, respectively. CONCLUSIONS： The method is proved to be simple and accurate, and it provides a more comprehensive scientific basis for the quality evaluation of Shenqi wuweizi capsule.