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Development of a miniaturised microarray-based assay for the rapid identification of antimicrobial resistance genes in Gram-negative bacteria |
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| Authors: | Batchelor Miranda Hopkins Katie L Liebana Ernesto Slickers Peter Ehricht Ralf Mafura Muriel Aarestrup Frank Mevius Dik Clifton-Hadley Felicity A Woodward Martin J Davies Rob H Threlfall E John Anjum Muna F |
| Affiliation: | aDepartment of Food and Environmental Safety, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK bLaboratory of Enteric Pathogens, Health Protection Agency Centre for Infections, London NW9 5EQ, UK cCLONDIAG, GmbH, Jena, Germany dDanish Institute for Food and Veterinary Research (DFVF), Copenhagen, Denmark eCentral Institute for Animal Disease Control Lelystad (CIDC-Lelystad), The Netherlands |
| Abstract: | We describe the development of a miniaturised microarray for the detection of antimicrobial resistance genes in Gram-negative bacteria. Included on the array are genes encoding resistance to aminoglycosides, trimethoprim, sulphonamides, tetracyclines and β-lactams, including extended-spectrum β-lactamases. Validation of the array with control strains demonstrated a 99% correlation between polymerase chain reaction and array results. There was also good correlation between phenotypic and genotypic results for a large panel of Escherichia coli and Salmonella isolates. Some differences were also seen in the number and type of resistance genes harboured by E. coli and Salmonella strains. The array provides an effective, fast and simple method for detection of resistance genes in clinical isolates suitable for use in diagnostic laboratories, which in future will help to understand the epidemiology of isolates and to detect gene linkage in bacterial populations. |
| Keywords: | Antimicrobial resistance Arrays Salmonella Escherichia coli |
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