VEGF在恶性淋巴瘤的表达及与MVD的相关性研究

目的探讨血管内皮生长因子(VEGF)在恶性淋巴瘤的表达,及与微血管密度(MVD)的关系。方法采用免疫组化法对恶性淋巴瘤淋巴结病理组织进行免疫学分类,及测定VEGF及微血管密度。结果(1)恶性淋巴瘤组织VEGF高表达,微血管密度增高,与对照组比较差异显著(P<0.01);(2)VEGF主要表达于淋巴瘤淋巴结外带,于肿瘤细胞及其周围细胞胞浆、血管内皮细胞及微血管周围表达;⑶非霍奇金恶性淋巴瘤VEGF与MVD呈正相关(P<0.01)。结论恶性淋巴瘤形成时已有VEGF高水平表达及丰富的微血管形成;VEGF由瘤细胞及周围组织细胞分泌,沉积于血管内皮细胞周围;非霍奇金淋巴瘤VEGF的分泌与微血管的形成相关。

The Expression of VEGF in Lymphoma and Its Relationship with MVD

Objective:To investigate the relationship between the expression of Vascular Endothelial Growth Factor(VEGF)and microvessels density(MVD)in Lymphoma.Methods:Immunohistochemical assay was performed with a SP kit to test the VEGF and microvessel density in lymphoma and normal lymphatic tissue.Results:(1)In lymphoma VEGF and MVD was significantly higher than control group (P<0.01);(2)VEGF was expressed in the outer band of lymph node,and was observed in the cytoplasm of tumor cells and their surrounding cells,as well as in the surrounding of microvessels and endothelials;(3)VEGF expression was correlated with MVD in NHL(P<0.01).Conclusion:VEGF and MVD is increased in lymphoma;VEGF is involved in the induction of the angiogenesis of lymphoma,which is secreted by tumor cells and surrounding cells;VEGF has a positive correlation with microvessel density in NHL.