针刺对D-半乳糖致亚急性衰老大鼠脑组织中NO、NOS及Ca2+-Mg2+-ATP酶活性的影响

目的:探讨针刺抗脑老化的作用机制.方法:将40只Wistar大鼠随机分为4组,每组10只.正常组10只不予任何处理.模型组、督穴组和体穴组大鼠复制D-半乳糖致急性衰老模型.模型组不予任何治疗;造模当日,督穴组予以针刺百会、大椎治疗,体穴组予以针刺关元、足三里治疗.观察各组大鼠脑组织中NO、NOS的含量及Ca2+-Mg2+-ATP酶活性.结果:造模成功后,大鼠脑组织中NO、NOS的含量明显升高,脑细胞膜Ca2+-Mg2+-ATP酶活性明显降低.针刺治疗有抑制NO、NOS含量升高和Ca2+-Mg2+-ATP酶活性降低的作用,督穴组强于体穴组(均P＜0.05).结论:针刺可以通过提高Ca2+-Mg2+-ATP酶活性,稳定NOS浓度,从而平衡NO含量,充分发挥NO的生理机能,而发挥抗脑衰老的作用,同时督穴组抗脑老化作用更为理想.

Effect of acupuncture on NO, NOS and Ca2+-Mg2+-ATPase activity in brain tissue of subacute aging rats induced by D-galactose

Objective: To explore the mechanism of acupuncture in treating brain aging. Methods: Forty healthy Wistar rats were randomly divided into 4 groups with 10 rats in each group. The rats in the control group didn’t received modeling and treatment. The rats in the model group, the Governor Vessel acupoints group (GVA group) and the body acupoints group (BA group) were induced as subacute aging rat model. The rats in the model group didn’t take treatment. On the modeling day, the rats in the GVA group received acupuncture at Baihui (GV 20) and Dazhui (GV 14) while the rats in the BA group received acupuncture at Guanyuan (CV 3) and Zusanli (ST 36). The contents of NO and NOS, as well as the activity of Ca 2+ -Mg 2+ -ATPase in the brain tissue of rats in each group were observed. Results: After modeling, the contents of NO and NOS of brain tissue increased significantly and the activity of Ca 2+ -Mg 2+ -ATPase decreased markedly. Acupuncture treatment could inhibit the increases of NO and NOS contents, improve the decrease of the activity of Ca 2+ -Mg 2+ -ATPase. The results of the GVA group were better than those of BA group (both P<0.05). Conclusion: Acupuncture improved the activity of Ca 2+ -Mg 2+ -ATPase and stabilized the NOS concentration. As a result, the NO content was balanced and the physiological function of NO was fully induced for anti-aging, especially in the GVA group.