Fluorographic study on the oxidative stress in the process of gastric mucosal injury: Attenuating effect of Vitamin E |
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Authors: | I. KUROSE D. FUKUMURA S. MIURA M. SUEMATSU M. SUZUKI E. SEKIZUKA H. NAGATA T. MORISHITA M. TSUCHIYA |
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Affiliation: | Department of Internal Medicine, School of Medicine, Keio University, Tokyo, Japan |
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Abstract: | Abstract In vivo oxidative change was visualized in the gastric mucosa of rats and the alteration was analysed by using a fluorescence microscope equipped with a digital imaging processor during the development of mucosal damage. Dichlorofluorescein (DCF)-associated fluorescence increased after the repeated electrical stimulation on the gastric artery (irritation), suggesting the occurrence of lipid peroxidation. The increase was enhanced in the mid-zone of two adjacent collecting venules. Allopurinol attenuated the oxidative stress in mucosa, showing the involvement of xanthine oxidase. Luminol-dependent chemiluminescence value in the blood taken from gastric vein was elevated by the irritation, suggesting that leucocyte-generated oxygen radicals also participate in this oxidative process. α-Tocopherol attenuated both the DCF activation and the increase in chemiluminescence value and prevented gastric mucosal injury. The present results suggest that α-tocopherol may be useful for the prevention of oxidative alteration in gastric mucosa. |
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Keywords: | free radicals gastric mucosal injury lipid peroxidation oxidative stress Vitamin E |
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