胃泌素/CCK-B受体环对多种肿瘤细胞生长和凋亡的影响 |
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引用本文: | 汪闯,周建奖,谢渊,赵艳.胃泌素/CCK-B受体环对多种肿瘤细胞生长和凋亡的影响[J].广东医学,2016(3):332-335. |
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作者姓名: | 汪闯 周建奖 谢渊 赵艳 |
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作者单位: | 贵州医科大学分子生物学重点实验室 贵阳550004 |
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基金项目: | 国家自然科学基金资助项目(编号81260303),贵州省2011协同创新中心项目(编号黔教合协同创新中心[2014]06),贵州省科学技术基金资助项目(编号黔科合J字[2012]2039号) |
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摘 要: | 目的:探讨胃泌素/CCK-B受体环对肝癌、肺癌和乳腺癌细胞生长和凋亡的影响。方法用免疫细胞化学检测肺癌A549细胞、肝癌HepG2细胞及乳腺癌MCF-7细胞系中胃泌素和CCK-B受体的表达。再用CCK-B受体拮抗剂丙谷胺(5 mmol/L)处理细胞(实验组),分别用MTT实验、流式细胞仪及分光光度法检测3种癌细胞的生长曲线、细胞凋亡及caspase-3的酶活性,对照组未用丙谷胺处理。结果肝癌HepG2细胞、肺癌A549细胞和乳腺癌MCF-7细胞中胃泌素和CCK-B受体的表达均为阳性;与对照组比较,实验组细胞的生长被显著抑制,caspase-3酶活性和凋亡细胞百分数显著升高,差异均有统计学意义(P<0.05,P<0.01)。结论胃泌素/CCK-B受体环参与肝癌、肺癌和乳腺癌细胞的生长和凋亡,阻断此环能抑制细胞的生长,促进细胞凋亡。
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关 键 词: | 胃泌素 CCK-B受体 肿瘤 细胞生长 细胞凋亡 |
Effects of gastrin/CCK -B receptor signaling on growth and apoptosis in selected cancer cell lines |
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Abstract: | Objective To investigate the effects of gastrin/CCK-B receptor signaling on cell growth and apopto-sis in liver cancer, lung cancer and breast cancer cell lines.Methods Expression of gastrin and CCK-B receptor was detected in hepatocarcinoma HepG2 cells, lung carcinoma A549 cells and breast cancer MCF-7 cells by immunocyto-chemistry.After treatment of 5 mmol/L proglumide (experimental groups), a CCK -B receptor antagonist, the cell growth curve, apoptosis and caspase-3 activity were detected by MTT assay, flow cytometry and spectrophotometry, re-spectively.The control groups were not treated with proglumide.Results The liver cancer HepG2 cells, lung cancer A549 cells and breast cancer MCK-7 cells showed positive expression for gastrin and CCK-B receptor.After proglumide treatment, cell growth was significantly inhibited, and caspase-3 activity and percentage of apoptotic cells significantly increased in all of the experimental groups compared with the control groups (P<0.05 or P<0.01).Conclusion Gas-trin/CCK-B receptor signaling is involved in the cell growth and apoptosis in hepatocarcinoma, lung carcinoma and breast cancer and blocking of the signal pathway can inhibit cell growth and promote apoptosis. |
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Keywords: | gastrin CCK-B receptor cancer cell growth apoptosis |
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