支气管哮喘患者诱导痰中白细胞介素-9水平及其与气道炎症的关系

目的探讨支气管哮喘患者诱导痰中白细胞介素-9（IL-9）的水平及其与炎症细胞和肺功能的关系。方法分别选择28例支气管哮喘急性发作患者（哮喘急性发作组）和28例健康对照（健康对照组）,分别检测哮喘急性发作期和治疗后缓解期及健康对照组的肺功能;并用酶联免疫吸附实验（ELISA）测定诱导痰上清液中IL-9的水平。结果哮喘发作组患者诱导痰中IL-9水平明显高于哮喘缓解期,比较差异有统计学意义（P〈0.05）,哮喘发作组和缓解期组痰中IL-9水平高于健康对照组,比较差异有统计学意义（P〈0.05）。哮喘发作组患者诱导痰中IL-9水平与中性粒细胞数及嗜酸性粒细胞数均呈正相关（r值分别为0.68、0.81,P〈0.05）,与第一秒用力呼气容积占预计值的百分比（FEV%）呈负相关（r=-0.65,P〈0.05）。结论支气管哮喘患者诱导痰中增高的IL-9水平与其气道炎症和气流受限有关。

The levels of interleukin 9 in induced sputum in patients with asthma and its relationship to airway inflammation

Objective To investigate the levels of interleukin-9（IL-9） in induced sputum in patients with asthma.and its relationship to the number of inflammation cells and lung function.Methods 28 patients with asthma and 28 normal control subjects were included in this study.Lung function was measured.Induced sputum was obtained and processed for cell differential and the supernatant was assayed for the concentrations of IL-9 by ELISA.Results The levels of IL-9 in induced sputum of the asthmatics were significantly higher than those in the controls and in normal control subjects（P 〈0.05） .In the acute exacerbated asthmatics,a positive correlation was found between the level of IL-9 and the percentage of neutrophile（r = 0.68,P 〈0.05）,but the level of IL-9 correlated inversely with FEV1%（r =-0.65,P〈 0.05） .Conclusion Increased levels of IL-9 in induced sputum in patients with asthma（especially in exacerbation） are associated with air-way inflammation and airflow limitation.