饱和盐水比浊法测定血浆纤维蛋白原 |
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引用本文: | 周筱梅 邓福贵. 饱和盐水比浊法测定血浆纤维蛋白原[J]. 四川省卫生管理干部学院学报, 2000, 19(1): 13-14 |
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作者姓名: | 周筱梅 邓福贵 |
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作者单位: | [1]四川省伤残军人医院检验科 [2]四川省卫生管理干部学院 |
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摘 要: | 目的:建立一种用饮和盐水比浊法测定血浆纤维蛋白原的方法。方法:用饱和盐水使血浆纤维蛋白原产生浊度,通过比浊测定血浆纤维蛋白原浓度,并进行方法学评价和对照试验,结果:该法线性好y=20.0673x-0.0224,r=0.999,批内重复试验CV=2.5%,批间重复试验CV=3.0%,平均干扰率〈5%,回收率为97%。与双缩脲法比较r=0.9194,y=0.9781x-0.4002有较高的一致性。结论
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关 键 词: | 血浆纤维蛋白原 饱和盐水 比浊法 测定 |
Saturated Salt Solution Turbidimetry Determined Fibrinogen in Plasma |
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Abstract: | Objective:To establish one method for determining fibrinogen in plasma by saturated salt solution turbidimetry.Method:We made the fibrinogen turbidty with saturated salt solution and determined the fibrinogen by turbidimetery.Then did methodological evaluation and comparison.Result:The linearity was very good.y=20.0673x 0.0224. r =0.9999.The within run cv was 2.5%.The between run cv was 3.0%.Average interfere rate was lower than 5%.The recovery rate was 97%.Compared turbidimetry with Biuret,We established the following regressive equation:y=0.9781x 0.4002. r =0.9194.Conclusion:Turbidimetry is simpler and easy to master.The determined results are satisfactory.Therefore,It can be used as one method for determining fibrinogen in plasma in clinical. |
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Keywords: | Fibrinogen in plasma Saturated salt solution Turbidimetry |
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