异丙酚对大鼠机械通气致肺损伤细胞因子表达的影响

目的 观察异丙酚对大鼠机械通气所致肺损伤及支气管肺泡灌洗液（BALF）肿瘤坏死因子α（TNF-α）、白介素-1 β（IL-1 β）水平的影响.方法 选择成年SD大鼠30只,随机分为对照组（C组）、机械通气组（V组）和机械通气＋异丙酚组（P组）,每组10只.C组以10 ml/kg潮气量进行机械通气,V组和P组设定潮气量为25 ml/kg以建立机械通气肺损伤模型.C组和V组在机械通气的同时输注5%葡萄糖溶液10 ml·kg-1·h-1,P组输注0.04%异丙酚10 ml·kg-1·h-1.机械通气4h后观察各组动物的肺湿/干重比值及BALF肿瘤坏死因子α（TNF-α）和白介素-1 β（IL-1 β）的浓度.结果 V组肺湿/干重比值以及BALF中TNF-α、IL-1 β的浓度均高于C组,P组肺湿/干重比值以及BALF中TNF-α、IL-1β的浓度均低于V组,与C组接近.结论 对大鼠进行25 ml/kg机械通气的同时输注0.04%异丙酚10 ml·kg-1·h-1,可以减轻机械通气所致肺损伤,其中的机制可能与抑制TNF-α、IL-1β等前炎症因子的释放有关.

Effect of propofol on expressions of cytokines in ventilation-induced lung injury

Objective To explore the effect of propofol on the expressions of cytokines in ventila- tion-induced lung injury. Methods Thirty Sprague Dawley rats were randomly divided into three groups with 10 in each. Group C received 10 ml ·kg-1·h-1 of 5% glucose infusion while was ventilated with a tidal volume of 10mlAg· Group V was infused with glucose at the same dose with a tidal volume of 25 ml/kg. Group P received 10 ml· kg-1·h-1 of 0.04% propofol infusion and was ventilated with a tidal volume of 25 ml/kg. All the rats were sacrified 4 hours after infusion. The wet/dry lung weight ratio and levels of TNF- α and IL-1β in the bronchoalveolar lavage fluid were measured. Results The wet/dry lung weight ratio and levels of TNF- a and IL- 1 β ere significantly increased in group V as compared with those in group C (P< 0. 05). Propofol significantly reduced the wet/dry lung weight ratio and levels of TNF-α and IL-1 β in group P as compared with group V. Conclusions Propofol inhibits the increase of the wet/dry lung weight ratio and levels of TNF- α. and IL-1 β in the bronchoalveolar lavage fluid after high tidal volume ventilation.